Immunofluorometric method and apparatus for measuring minute qua

Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure

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23230B, 23915, 250458, 250461B, 1951035A, 424 12, 422 56, G01N 2152, G01N 3316

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active

041536754

ABSTRACT:
An apparatus for measuring minute quantities of antigens, antibodies and other substances characterized by binding reactions with one another for forming a sandwich constituting an inner layer having a distribution of an unlabelled reagent, an intermediate layer having a distribution of the substance to be measured and an outer layer having a distribution of a fluorescent labelled reagent, the labelled and unlabelled reagents having the same biochemical specificity. Illumination from an excitation source is directed towards the sandwich and the fluorescence emitted by the fluorescent label is detected for measuring minute quantities of the substance under diagnosis.

REFERENCES:
patent: 3654090 (1972-04-01), Schuurs
patent: 3973129 (1976-08-01), Blumberg
patent: 3992631 (1976-11-01), Harte
patent: 4036946 (1977-07-01), Kleinerman

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