Methods and reagents for the rapid determination of glycated hem

Details Methods and reagents for the rapid determination of glycated hem Methods and reagents for the rapid determination of glycated hem

1995-04-28

1997-11-11

Chin, Christopher L.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

435 325, 435975, 436 8, 436 16, 436179, 436520, 436522, 436531, 436533, 436534, 436536, 436538, 436541, 436826, 436805, 436827, G01N 33543, G01N 33545, G01N 33537, G01N 33538

Patent

active

056863160

ABSTRACT:
The invention is a rapid, continuous test for glycated hemoglobin using a non-equilibrium affinity binding method. Agarose beads derivatized with 3-aminophenylboronic acid specifically bind glycated hemoglobin. This solid phase is incorporated into a sample processor card, modified to mix and to separate the test solution from the solid phase prior to absorbance readings. Two absorbance readings are made on the test solution, one immediately after mixing the reagent/diluent with the specimen, and one after a significant amount of binding has occurred. A linear correlation between total glycated hemoglobin and hemoglobin A.sub.1c permits standardization and reporting of units equivalent to % hemoglobin A.sub.1c. Stable glycated hemoglobin solutions for use as standards in the assay, and a method for preparing the standards are also disclosed.

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