Method and composition for testing blood for hemoglobin S that i

Details Method and composition for testing blood for hemoglobin S that i Method and composition for testing blood for hemoglobin S that i

1995-02-13

1996-05-07

Warden, Robert J.

Chemistry: analytical and immunological testing

Hemoglobin, myoglobin, or occult blood

436 8, 422 61, 435 2, 435810, G01N 3372

Patent

active

055145922

ABSTRACT:
A method for testing blood for the presence of hemoglobin S wherein a blood sample is vigorously admixed with a two-phase test system for the purpose of determining the presence or absence of hemoglobin S after separation of the phases and wherein the two-phase test system involves an aqueous phase containing a lysing agent, a chemical reducing system and a phosphate ion buffer system; and an immiscible phase of mineral oil. The hemoglobin S presence is determinable by the color of a band formed at the interface of the aqueous and immiscible phases and wherein the invention further includes a composition suitable for testing whole blood for hemoglobin S.

REFERENCES:
patent: 3492095 (1970-01-01), Tillem
patent: 3607695 (1971-09-01), Schneider
patent: 3761226 (1973-09-01), Louderback et al.
patent: 3847482 (1974-11-01), Sokol et al.
patent: 3847545 (1974-11-01), Shanbrom et al.
patent: 3918905 (1975-11-01), Warren et al.
patent: 4981819 (1991-01-01), Rinn
The Condensed Chemical Dictionary--10th edition, 1981, p. 1030.
Bowie et al. Clinical Chemistry, vol. 29, No. 2, 1983, pp. 325-328.
Warren et al. American Journal of Medical Technology, vol. 41, No. 9, Sep. 1975, pp. 317-321.

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