Surfactant-enhanced light emission- or absorbance-based binding

Details Surfactant-enhanced light emission- or absorbance-based binding Surfactant-enhanced light emission- or absorbance-based binding

1994-07-26

1995-11-14

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436164, 436172, 935 77, 935 78, C12Q 168, G01N 2164

Patent

active

054665787

ABSTRACT:
The fluorescence of polycyclic aromatic labels, and excimers of these lab, attached to nucleic acids is greatly enhanced by the presence of quaternary ammonium surfactants having at least one long chain (C4 or greater) alkyl group. This enchancement may be advantageously used in Pi Overlapping Rings Systems Contained in a Homogeneous Assay (PORSCHA) and in conventional assays.

REFERENCES:
patent: 5279940 (1994-01-01), Kissel
patent: 5332659 (1994-07-01), Kidwell
Kitamura, Nuclei Acid Research (1991) 67-68.
Jiang et al Huaxue Xuebao (1991) 149:854.

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