Methods of expressing proteins in insect cells and methods of ki

Details Methods of expressing proteins in insect cells and methods of ki Methods of expressing proteins in insect cells and methods of ki

1997-09-16

1999-11-23

Guzo, David

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

424 936, 435 691, 4351723, 4353201, A61K 4800, C12N 1586, C12N 1567

Patent

active

059895411

ABSTRACT:
Described herein is a method of expressing heterologous proteins in insect cells using an expression cassette comprising a structural gene for a heterologous protein physically attached to an insect cellular promoter and an enhancer. The cells may also express the IE-1 product. Also described herein is a method of killing insects comprising infecting the insects with a recombinant baculovirus comprising a structural gene for an incompatible protein functionally linked to an insect cellular promoter and an enhancer. The invention is also directed towards expression cassettes comprising an insect cellular promoter functionally linked to an enhancer wherein the promoter is capable of directing the expression of a heterologous protein in tissues containing the expression cassette, recombinant expression cassettes containing heterologous proteins, transplacement fragments, vectors and recombinant baculoviruses.

REFERENCES:
patent: 4745051 (1988-05-01), Smith et al.
patent: 5004687 (1991-04-01), Miller
patent: 5071748 (1991-12-01), Miller
patent: 5077214 (1991-12-01), Guarino et al.
patent: 5091179 (1992-02-01), Biache et al.
patent: 5093119 (1992-03-01), Hickle et al.
patent: 5093120 (1992-03-01), Edwards et al.
patent: 5104974 (1992-04-01), Sick et al.
patent: 5133962 (1992-07-01), Sick et al.
patent: 5147788 (1992-09-01), Page et al.
patent: 5155037 (1992-10-01), Summers
patent: 5169784 (1992-12-01), Summers et al.
patent: 5186933 (1993-02-01), Estes
patent: 5194376 (1993-03-01), Kang
patent: 5322774 (1994-06-01), Peakman et al.
Blissard and Rohrmann (1990) "Baculovirus diversity and moleclar biology" Ann. Rev. Entomol., 35:127-155.
Bradford (1976) "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding" Anal. Biochem., 72:248-254.
Carbonell et al. (1988) "Synthesis of a gene coding for an insect-specific scorpion neurotoxin and attempts to express it using baculovirus vectors," Gene, 73:409-418.
Carson et al., (1988) "Functional Mapping of an AcNPV Immediately Early Gene which Augments expression of the IE-1 trans-activated 39K Gene" Virol. 162:444-451.
Carson et al. (1991) "Transient expression of the Autographa californica nuclear polyhedrosis virus immediate-early gene, IE-N, is regulated by three viral elements" J. Virol. 65:945-951.
Cochran and Faulkner (1983) "Location of homologous DNA sequences interspersed at five regions in the baculovirus AcMNPV genome", J. Virol. 45:961-970.
Fotaki and Iatrou (1988) "Identification of a Transcriptionally Active Pseudogene in the Chorion Locus of the Silkmoth Bombyx mori," J. Mol. Biol., 203:849-860.
Gorman et al. (1982) "Recombinant Genomes which express chloramphenicol acetyltransferase in mammalian cells," Mol. Cell Biol., 2:1044-1051.
Goswami and Glazer (1991) "A simplified method for the production of recombinant baculovirus," BioTechniques, 10:626-630.
Grace (1967); "Establishment of a line of cells from the silkworm Bombyx mori," Nature 216:613.
Granados and Lawler (1981) "In vivo pathway of Autographa californica baculovirus invasion and infection," Virol., 108:297-308.
Guarino and Dong (1991) "Expression of an enhancer-binding protein in insect cells transfected with the Autographa californica nuclear polyhedrosis virus IE-1 gene" J. Virol. 65:3676-3680.
Guarino et al., (1986) "Complete sequence and enhancer function of the homologous DNA regions of Autographa californica nuclear polyhedrosis virus" J. Virol. 60:224-229.
Guarino and Summers (1986) "Interspersed homologous DNA of Autographa californica nuclear polyhedrosis virus enhances delayed-early gene expression" J. Virol. 60:215-223.
Guarino and Summers, (1987) "Nucleotide sequence and temporal expression of a baculovirus regulatory gene" J. Virol. 61:2091-2099.
Gubler and Hoffman (1983) "A simple and very efficient method of generating cDNA libraries," Gene, 25:263-269.
Gurevitz et al. (1991) "Nucleotide sequence and structure analysis of a cDNA encoding an alpha insect toxin from the scorpion Leiurus quinquestriatus hebraeus", Toxicon 29:1270-1272.
Hahnel and Schultz (1989) "Cloning and characterization of a cDNA encoding alkaline phosphatase in mouse embryonal carcinoma cells," Clin, Chim. Acta, 186:171-174.
Hanzlik et al. (1990) "Isolation and sequencing of cDNA clones coding for juvenile hormone esterase from Heliothis virescens," J. Biol. Chem., 264:12419-12425.
Huber (1986) "Use of baculoviruses in pest management programs," in The Biology of Baculoviruses, vol. II Granados and Federici, Eds., CRC Press, Boca Raton, Fl. pp. 181-202.
Huybrechts et al., (1992) "Nucleotide sequence of a transactivating Bombyx mori nuclear polyhedrosis virus immediate early gene" Biochim. Bioph. Acta 1129:328-330.
Iatrou et al. (1985) "Polyhedrin gene of Bombyx mori nuclear polyhedrosis virus," Journal of Virology, 54:436-445.
Iatrou et al. (1988) "Towards the development of a vector for transducing homologous and foreign genes into silkmoth tissue culture cells and animals," in Endoctrinological Frontiers in Physiological Insect Ecology, Eds. Sehnal et al., Wroclaw Technical University Press, Wroclaw, pp. 1055-1073.
Iatrou et al. (1989) "Recombinant baculoviruses as vectors for identifying proteins encoded by intron-containing members of complex multigene families," Proc. Natl. Acad. Sci. USA, 86:9129-9133.
Iatrou and Meidinger (1989) "Bombyx mori nuclear polyhedrosis virus-based vectors for expressing passenger genes in silkmoth cells under viral or cellular promoter control," Gene, 75:59-71.
Iatrou and Meidinger (1990) "Tissue-specific expression of silkmoth chorion genes in vivo using Bombyx mori nuclear polyhedrosis virus as a transducing vector," Proc. Natl. Acad. Sci. USA, 87:3650-3654.
Iatrou et al. (1992) "Recombinant baculoviruses for lepidopteran insect pest control," Sericologia 31, Suppl. 78 [Abstract].
Johnson et al. (1992) "A Cellular Promoter-Based Expression Cassette for Generating Recombinant Baculoviruses Directing Rapid Expression of Passenger Genes in Infected Insects," Virology, 190:815-823.
Kamita et al., (1993) "Identification and characterization of the p35 gene of Bombyx mori nuclear polyhedrosis virus that prevents virus-induced apoptosis" J. Virol. 67:455-463.
Kang, C.Y. (1988) "Baculovirus vectors for expression of foreign genes," Adv. Virus Res. 35:177-192.
Kang, C.Y. "Improved Baculovirus Expression System Capable of Producing Foreign Gene Proteins at High Levels," University of Ottawa Fact Sheet.
Keddie et al. (1989) "The pathway of infection of Augographa californica nuclear polyhedrosis virus in an insect host." Science 243:1728-1730.
Kool et al., (1993) "Location of two putative origins of DNA replication of Autographa californica nuclear polyhedrosis virus" Virology 192:94-101.
Kovacs et al. (1991) Novel regulatory properties of IE1 and IE0 transactivators encoded by the baculovirus Autographa californica multicapsid nuclear polyhedrosis virus, J. Virol., 65:5281-5288.
Kunkel (1985) "Rapid and efficient site-specific mutagenesis without phenotypic selection," Proc. Natl. Acad. Sci. USA, 82:488-492.
Maeda et al. (1991) "Insecticidal effects of an insect-specific neutrotoxin expressed by a recombinant baculovirus," Virology, 184:777-780.
Maeda (1989) "Expression of foreign genes in insects using baculovirus vectors," Ann. Rev. Entomol., 34:351-372.
Maeda (1989) "Increased insecticidal effect by a recombinant baculovirus carrying a synthetic diuretic hormone gene," Biochem. Biophys. Res. Commun., 165:1177-1183.
Maeda and Majima (1990) "Molecular cloning and physical mapping of the genome of Bombyx mori nuclear polyhedrosis virus" J. Gen. Virol. 71:1851-1855.
Majima et al. (1993) "Divergence and evolution of homologous regions of Bombyx mori nuclear polyhedrosis virus" J. Virol. 67:7513-7521.
Maxam and Gilbert (1977) " A new method for sequencing DNA," Proc. Natl. Acad. Sci. USA, 74:560-564.
McCutcheon et al. (1991) "Development of a recombinant baculovirus expressing an insect-selective neurotoxin: potential for pest control," Bio/Technology, 9:848-852.
Merryweather et al. (1

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