Nucleic acid molecules encoding non-infectious, non-replicating,

Details Nucleic acid molecules encoding non-infectious, non-replicating, Nucleic acid molecules encoding non-infectious, non-replicating,

1996-12-06

1999-03-30

Scheiner, Laurie

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 691, 4352351, 435256, 4353201, C07H 2104, C12N 1500, C12N 704, C12N 700

Patent

active

058891765

ABSTRACT:
Non-infectious, retrovirus-like particles comprise an assembly of an env gene product, a pol gene product and a gag gene product contain an antigenic marker which is non-retroviral or non-HIV retroviral. In one embodiment, the marker comprises an amino acid sequence containing an epitope inserted into the gag gene product at an antigenically-active insertion site. In another embodiment, the marker comprises an antigenic anchor sequence operatively connected to the env gene product replacing endogenous anchoring function. The corresponding nucleic acid molecules are described. The non-infectious, retrovirus-like particles have utility in in vivo administration including to humans and in diagnosis. The presence of the antigenic marker enables recognition that antiserum containing anti-retroviral antibodies has been generated by exposure to the non-infectious retrovirus-like particles by testing for antibodies specific to the antigenic marker.

REFERENCES:
Rovinski, B., Haynes, J.R., Cao, S.X., James, O., Sla, C., Zolla-Pazner, S., Matthews, T.J. and Klein, M. (1992) J. Virol., 66, 4003-4012.
Wain-Hobson, S., Sonigo, P., Danos, O., Col, S. and Alizon, M. (1985) Cell, 40, 9-17.
Myers, G., Berzofsky, J.A., Rabson, A.B., Smith, T.F. and Wong-Staal, F. (ed.) (1990) Human retroviruses and AIDS. Theoretical Biology and Biophysics, Group T-10. Los Alamos National Laboratory, Los Alamos, N.Mex.
Alizon, M., Sonigo, P., Barre-Sinoussi, F., Chermann, J.C., Tiollais, P., Montagnier, L. and Wain-Hobson, S. (1984) Nature, 312, 757-780.
Min Jou, W., Verhoeyen, M., Devos, R., Saman, E., Fang, R., Huylebroeck, D. and Fiers, W. (1980) Cell, 19, 683-696.
Westhof, E., Altschuh, D., Moras, D., Bloomer, A.C., Mondragon, A., Klug, A. and Van Regenmortel, M.H. (1984) Nature, 311, 123-126.
Trifilleff, E., Dubs, M.C. and Regenmertel, M.H.V. (1991) Mol. Immunol., 28, 889-896.
Ulmer et al., 1993 current Drugs Ltd. ISSN 0967-8298.
Hunter, 1994, Sem. Virol. 5:71.
Karacostas et al. 1993, Virol. 193: 661-671.
Rovinski et al., 1992 J. Virol. 66: 4003-4012.
Perkins et al., 1991, J. Immunol. 146: 2137-2144.
Shama et al. 1993, Vaccine 11: 1321-1326.
Haynes et al. 1991, AIDS Res. Human Retro. 7:17-27.
Zhao-Wen, 1980, Nuc. Acids Prot. Proc Symp. 196-200.
Klein M., Aids Research and Human Retroviruses--vol. 10, Supplement 1, Aug. 1994--"Neutralizing activities of HIV-1 pseudovirions and T-B tandem epitopes".
Haynes J.R. et al., The vaccine Symposium, Toronto, Ontario, Canada, Oct. 1989. Molecular Immun. 28(3) 1991. pp. 231-234, "Strategy for Developing a Genetically-Engineered Whole-Virus Vaccine against HIV".
Karacostas, V. et al. Proceedigns of the National Academy of Sciences of the USA, vol. 86, No. 22 Nov. 1, 1989, "Immunodeficiency Virus-Like Particles Produced by a Vaccinia Virus Expression Vector".
Yao., Fei-Long et al. Biotechniques (1995), 18(3) pp. 372-374--Mar. 1995, Gene assembly-aided mutagenesis (GAAM).
Hunter, E., 1994, "Macromolecular interactions in the assembly of HIV and other retroviruses", Sem. Virol. 5:71-83.
Karacostas et al., 1989, "Human immunodeficiency virus-like particles produced by a vaccinia virus expression vector", Proc. Natl. Acad. Sci. USA 86:8964-8967.
Rovinski et al., 1992, "Expression and characterization of genetically engineered human immunodeficiency virus-like particles containing modified envelope glycoproteins: implications for development of a cross-protective AIDS vaccine", J. Virol. 66(7):4003-4012.
Sharma et al., 1993, "Co-dominant and reciprocal T-helper cell activity of epitopic sequences and formation of junctional B-cell determinants in synthetic T:B chimeric immunogens", Vaccine 11(13):1321-1326.
Perkins et al., 1991, "Immunodominance intramolecular competition between T cell epitopes", J. Immunol. 146(7):2137-2144.
Coffin, J., 1996, "Retroviridae: the viruses and their replication", in Fields Virology, Third Edition, Fields et al., eds., Lippincott-Raven Publishers, Philadelphia, pp. 1767-1771.

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