Integral multilayer analytical element for determination of ammo

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving urea or urease

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435 17, 435805, 422 56, 422 57, 436169, 436170, 526332, C12Q 158, C12Q 150, G01N 2177

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active

052866246

ABSTRACT:
An integral multilayer analytical element for the determination of ammonia or an ammonia-producing substance comprising a light-transmissive liquid-impermeable support, an indicator layer containing an indicator which produces a detectable change by gaseous ammonia, a liquid permeation barrier layer, a reagent layer containing an alkaline buffer and optionally a reagent capable of reacting with a substance to produce ammonia and a spreading layer laminated in this order, which is improved by that the indicator layer contains a polyvinyl alkyl ether, and/or which is improved by that the surface of said support facing toward the indicator layer is undercoated with a polyvinyl alkyl ether, a hydroxyalkyl cellulose, an alkyl cellulose, polystyrene, a polyalkyl methacrylate, polyviriylidene chloride, polyvinyl alcohol or polyvinyl pyrrolidone, substantially not containing ammonia and ammonium ion. By using the above analytical element, ammonia or an ammonia-producing substance can be analyzed at a high coloring optical density and a high accuracy. The measuring accuracy is further improved by lowering the background optical density.

REFERENCES:
patent: 2799669 (1957-07-01), Zoss
patent: 4557901 (1985-12-01), Koyama
patent: 4604347 (1986-08-01), Arai
patent: 4895704 (1990-01-01), Arai
Encyclopedia of Polymer Science & Engineering vol. 17, John Wiley & Sons N.Y. 1989, pp. 456-457.

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