Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-06-07
1998-07-07
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
530350, C12N 916
Patent
active
057767521
ABSTRACT:
The present invention relates to novel purified and isolated nucleotide sequences encoding mammalian Ca.sup.2+ /calmodulin stimulated phosphodiesterases (CaM-PDEs) and cyclic-GMP-stimulated phosphodiesterases (cGS-PDEs). Also provided are the corresponding recombinant expression products of said nucleotide sequences, immunological reagents specifically reactive therewith, and procedures for identifying compounds which modulate the enzymatic activity of such expression products.
REFERENCES:
Ausubel, et al., eds., Current Protocols in Molecular Biology, 1: 1.7.1-1.7.2 and 9.2.1-9.2.3, John Wiley & Sons, New York (1989).
Beavo, J.A., "Multiple Isozymes of Cyclic Nucleotide Phosphodiesterase," Advances in Second Messenger and Phosphoprotein Research, 22: 1-38 (1988).
Beavo, J.A., "Multiple Phosphodiesterase Isoenzymes Background, Nomenclature and Implications", pp. 3-15; Cyclic Nucleotide Phosphodiesterases; Structure, Regulation and Drug Action, J. Beavo and Houslay, M.D., Eds.; John Wiley & Sond, Ltd., New York (1990).
Birnstiel, M.L., et al., "Transcription Termination and 3' Processing: The End Is in Sight|", Cell, 41: 349-359 (1985).
Bourne, H.R., et al., "Somatic Genetic Analysis of Cyclic AMP Action: Characterization of Unresponsive Mutants," J. Cell. Physiol., 85:611-620 (1985).
Bradford, M.M., "A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding," Analytical Biochem., 72:248-254 (1976).
Chen, C-N., et al., "Molecular Analysis of cDNA Clones and the Corresponding Genomic Coding Sequences of the Drosophila dunce .sup.+ Gene, the Structural Gene for cAMP Phosphodiesterase," Proc. Nat'l. Acad. Sci. (USA), 83:9313-9317 (1986).
Chomczynski, P., et al., "Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction," Analytical Biochem., 162:156-159 (1987).
Coliceli, J., et al., "Isolation and Characterization of a Mammalian Gene Encoding a High-Affinity cAMP Phosphodiesterase," Proc. Nat'l. Acad. Sci. (USA), 86:3599-3603 (1989).
Davis, R.L., "Molecular Genetics of the Cyclic Nucleotide Phosphodiesterases", pp. 227-241 in Cyclic Nucleotide Phosphodiesterases: Structure, Regulation and Drug Action, J. Beavo and Houslay, M.D., Eds.; John Wiley & Sons, Ltd., New York (1990).
Davis, R.L., et al., "Cloning and Characterization of Mammalian Homologs of the Drosophila dunce .sup.+ Gene," Proc. Nat'l. Acad. Sci. (USA), 86:3604-3608 (1989).
Devereux, J., et al., "A Comprehensive Set of Sequence Analysis Programs for the VAX," Nucleic Acids Res., 12:387-395 (1984).
Erneux, C., et al., "A Mechanism in the Control of Intracellular cAMP Level: The Activation of a Calmodulin-Sensitive Phosphodiesterase by a Rise of Intracellular Free Calcium," Mol. Cell. Endocrinol., 43:123-134 (1985).
Faure, M., et al., "Disruption of Dictyostelium discoideum Morphogenesis by Overproduction of cAMP Phosphodiesterase," Proc. Nat'l. Acad. Sci. (USA), 85:8076-8080 (1988).
Feinberg, A.P., et al., "A Technique for Radiolabeling DNA Restriction Endonuclease Fragments to High Specific Activity," Analytical Biochem., 137:266-267 (1984).
Greenberg, L.H., et al., "Enzymatic Regulation of the Concentration of Cyclic GMP in Mouse Brain," Neuropharmacology, 17:737-745 (1978).
Hansen, R.S., et al., "Differential Recognition of Calmodulin-Enzyme Complexes by a Conformation-Specific Anti-Calmodulin Monoclonal Antibody," J. Biol. Chem., 261:14636-14645 (1986).
Hansen, R.S., et al., "Purification of Calmodulin-Stimulated Cyclic Nucleotide Phosphodiesterase by Monoclonal Antibody Affinity Chromatography," Meth. Enzymol., 159:543-557 (1988).
Hansen, R.S., et al., "Purification of Two Calcium/Calmodulin-Dependent Forms of Cyclic Nucleotide Phosphodiesterase by Using Conformation-Specific Monoclonal antibody Chromatography," Proc. Nat'l. Acad. Sci. (USA), 79:2788-2792 (1982).
Hashimoto, Y., et al., "Regulation of Ca.sup.2+ / Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase by the Autophosphorylated Form of Ca.sup.2+ /Calmodulin-Dependent Protein Kinase II," J. Biol. Chem., 264:10884-10887 (1989).
Henikoff, S., "Unidirectional Digestion with Exonuclease III Creates Targeted Breakpoints for DNA Sequencing," Gene , 28:351-359 (1984).
Kincaid, R.L., et al., "Differential Localization of Calmodulin-Dependent Enzymes in Rat Brain: Evidence for Selective Expression of Cyclic Nucleotide Phosphodiesterase in Specific Neurons," Proc. Nat'l. Acad. Sci. (USA), 84:1118-1122 (1987).
Kozak, M., "The Scanning Model for Translation: An Update," J. Cell Biol., 108:229-241 (1989).
Krinks, M.H., et al., "Reversible and Irreversible Activation of Cyclic Nucleotide Phosphodiesterase: Seperation of the Regulatory and Catalytic Domains by Limited Proteolysis," Advances in Cyclic Nucleotide and Protein Phosphorylation Research, 16:31-47 (1984).
LaPorte, D.C., et al., "Cross-Linking of Iodine-125-Labeled, Calcium-Dependent Regulatory Protein to the Ca.sup.2 + -Sensitive Phosphodiesterase Purified from Bovine Heart," Biochemistry, 18:2820-2825 (1979).
LeTrong, H., et al., "Amino Acid Sequence of the Cyclic GMP Stimulated Cyclic Nucleotide Phosphodiesterase from Bovine Heart," Biochemisry, 29:10280-10288 (1990).
Livi, G.P., et al., "Cloning and Expression of cDNA for a Human Low-K.sub.m Rolipram-Sensitive Cyclic AMP Phosphodiesterase," Mol. Cell. Biol., 10:2678-2686 (1990).
Manganiello, V.C., et al., "Cyclic GMP-Stimulated Cyclic Nucleotide Phosphodiesterases", pp. 62-85 in Cyclic Nucleotide Phosphodiesterases: Structure, Regulation and Drug Action, Beavo, J. and Houslay, M.D., Eds,; John Wiley & Sons, Ltd., New York (1990).
Maniatis, et al., Molecular Cloning: A Laboratory Manual, pp. 324-328, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1982).
Martins, T.J., et al., "Purfication and Characterization of a Cyclic GMP-Stimulated Cyclic Nucleotide Phosphodiesterase from Bovine Tissues," J. Biol. Chem., 257:1973-1979 (1982).
Nikawa, J-I., et al., "Cloning and Characterization of the Low-Affinity Cyclic AMP Phosphodiesterase Gene of Saccharomyces cerevisiae, " Mol. Cell. Bio., 7:3629-3636 (1987).
Nomenclature Committee of the International Union of Biochemistry (NCIUB), "Nomenclature for Incompletely Specified Bases in Nucleic Acid Sequences," J. Biol. Chem., 261:13-17 (1986).
Novack, J.P., et al., "Sequence Comparison of the 63-, 61-, and 59-kDa Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterases," Biochemistry, 30:7940-7947 (1991).
Ovchinnikov, Y.A., et al., "Cyclic GMP Phosphodiesterase from Bovine Retina," FEBS, 223:169-173 (1987).
Sanger, F., et al., "DNA Sequencing with Chain-Terminating Inhibitors," Proc. Nat'l. Acad. Sci. (USA), 74:5463-5467 (1977).
Sass, P., et al., "Cloning and Characterization of the HIgh-Affinity cAMP Phosphodiesterase of Saccharomyces cerevisiae," Proc. Nat'l. Acad. Sci. (USA), 83:9303-9307 (1986).
Seed, B., "An LFA-3 cDNA encodes a Phospholipid-Linked Membrane Protein Homologous to Its Receptor CD2," Nature, 329:840-842 (1987).
Sharma, R.K., et al., "Demostration of Bovine Brain Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase Isozymes by Monoclonal Antibodies," J. Biol. Chem., 259:9248-9254 (1984).
Sharma, R.K., et al., "Differential Regulation of Bovine Brain Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase Isozymes by Cyclic AMP-Dependent Protein Kinase and Calmodulin-Dependent Phosphatase," Proc. Nat'l. Acad. Sci. (USA), 82:2603-2607 (1985).
Sharma, R.K., et al., "Purification and Characterization of Bovine Lung Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase," J. Biol. Chem., 261:14160-14166 (1986).
Short, M., et al., "ZAP: A Bacteriophage .lambda. Expression Vector with in vivo Excision Properties," Nucleic Acids Res., 16:7583-7600 (1988).
Sonnenburg, W.K., et al., "Molecular Cloning of a Cyclic GMP-Stimulated Cyclic Nucleotide Phosphodiesterase cDNA," J. Biol. Chem., 266(26):17655-17661 (1991).
Stroop, S.D., et al., "Direct Photolabeling of the cGMP-Stimulated Cyclic Nucleotide Phosphodiesterase," J. Biol. Chem., 264:13718-13725 (1989).
Swinnen, J.V., et
Beavo Joseph A.
Bentley J. Kelley
Charbonneau Harry
Sonnenburg William K.
Longton Enrique E.
The Board of Regents of the University of Washington
Wax Robert A.
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