Method for preparation and analysis of leukocytes in whole blood

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435808, 436526, 436171, 436800, 436805, 356336, 356338, G01N 33533

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active

057767092

ABSTRACT:
A method of flow cytometric analysis of leukocyte subpopulations using a fluorescence trigger and gating on light scatter vs. fluorescence. The methods are useful where light scatter parameters are unsatisfactory for identification of leukocyte subpopulations, for example when analyzing lysed blood samples without removal of lysing reagent or unbound label prior to analysis.

REFERENCES:
patent: 4284412 (1981-08-01), Hansen et al.
patent: 4654312 (1987-03-01), Chang et al.
patent: 4727020 (1988-02-01), Recktenwald
patent: 4751179 (1988-06-01), Ledis
patent: 4805225 (1989-02-01), Clark
patent: 4902613 (1990-02-01), Chang et al.
patent: 4965725 (1990-10-01), Rutenberg
patent: 4987086 (1991-01-01), Brosnan et al.
patent: 5017497 (1991-05-01), de Grooth et al.
patent: 5030554 (1991-07-01), Quintana et al.
patent: 5041733 (1991-08-01), Noguchi et al.
patent: 5047321 (1991-09-01), Loken et al.
patent: 5138170 (1992-08-01), Noguchi et al.
patent: 5144224 (1992-09-01), Larsen
Horan, et al. "Improved flow cytometric analysis of leukocyte subsets: Simultaneous identification of five cell subsets using two-color immunofluorescence" PNAS 83:8361-8365 (1986).
Begg, et al. "Cell Kinetic Analysis of Mixed Populations Using Three-Color Fluorescence Flow Cytometry" Cytometry 12:445-454 (1991).
R. P. Lippmann "An Introduction to Computing with Neural Nets" IEEE ASSP Magazine pp. 4-22, Apr. 1987.
F. F. Mandy, et al. "Automated Software for Rapid Analysis of List Mode Data From Simultaneous Three Color Flow Cytometry" Cytometry Suppl. 5:135 (1991) Abstract 741C.
F. F. Mandy, et al. "Cell Lineage Specific Gating Method for T Cell Subset Analysis" Cytometry Suppl. 5:79 (1991) Abstract 399C.
K. Shults, et al. "CD45 Antigen Density Versus RALS: New Tool for Analysis for Blood and Bone Marrow" Cytometry Suppl. 5:79 (1991) Abstract 400D.
C. W. Caldwell, et al. "A Rapid, No-Wash Technic for Immunophenotypic Analysis by Flow Cytometry" Am. J. Clin. Pathol. 86:600-607 (1986).
R. A. Hoffman "Immunofluorescence Analysis of Leukocytes Without Red Cells Lysis" Cytometry Suppl. 1:36 (1987) Abstract 172.
L.W.M.M. Terstappen, et al. "A Rapid Sample Preparation Technique for Flow Cytometric Analsysis of Immunofluorescence Allowing Absolute Enumeration of Cell Populations" J. Immunol. Mtds. 123:103-112 (1989).
M.R. Loken, et al. "Establishing Optimal Lymphocyte Gates for Immunophenotyping by Flow Cytometry" Cytometry 11:453-459 (1990).
Ortho Diagnostic Systems, Inc. "Direct Immunofluorescence Prodedure for Whole Blood Analysis of Leukocyte Subsets by Flow Cytometry" Published as a Laboratory Method by ODSI, Jan. 1985.
D. D. Egbert, et al. "Preprocessing of Biomedical Images for Neurocomputer Analysis" IEEE Int. Conf. on Neural Networks IEEE, pub., New York, N.Y. pp. 561-568 (1988).
Y. Imasato, et al. "CYBEST--Automated Pap Smear Prescreener" Toshiba Review (Intl. Ed.) n 100, pp. 60-63, Nov.-Dec. 1975.

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