Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-03-02
1998-07-07
Grimes, Eric
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435 28, 436164, 436172, C12Q 126, G01N 3353
Patent
active
057767033
ABSTRACT:
There is provided an assay of enhanced sensitivity for the qualitative and quantitative determination of haptens. These may be assayed entities such as antigens, antibodies, a wide variety of biologically active proteins. The novel assay of increased sensitivity is based on the formation of a complex of the entity to be measured with another entity, tagging this conjugate with a oxidizing entity, reacting the resultant entity with a redox substrate to form a product which is photosensitive and irradiating same thus enhancing the sensitivity of the assay. Colored or fluorescent products give highly sensitive results. According to a preferred embodiment the oxidized entity is reacted with a non-ionic detergent, enhancing the resulting fluorescence.
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Harlow et al (1988) Antibodies: A Laboratory Manual. ColdSpring Harbor Press. pp. 558 579-581.
Bystrak et al (1992) Anal. Biochem. 202 390-393.
Mekler et al (1992) Anal. Chim. Acta 264 359-363.
Baeyens et al (1989) J. Pharm. Biomed. Anal. 7 1385-1394.
Schaler et al (1989) J. Immunological Methods 116:27-29.
Bethesda Research Laboratories 1988 Catalogs p. 120.
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