Development of a PCR-based method for identification of Tilletia

Details Development of a PCR-based method for identification of Tilletia Development of a PCR-based method for identification of Tilletia

1996-12-24

1998-07-07

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 243, 935 76, 935 77, 935 78, C12Q 168, C07H 2102, C07H 2104, C12N 1500

Patent

active

057766860

ABSTRACT:
The polymerase chain reaction (PCR) was used to identify Tilletia indica, the causal agent of Karnal bunt of wheat. The method uses two sets of oligonucleotide primers developed by sequence analysis of cloned Dra I fragments of mitochondrial DNA of T. indica. The primer pair TI17M1 (5'-TCCCCTTGGATCAGAACGTA-3') and TI17M2 (5'-AGAAGTCTAACTCCCCCCTCT-3'), derived from clone pTI-MD17, amplified a single 825-bp product from all isolates of T. indica and no products for other Tilletia species. In addition, the primer pair TI57M1 (5'-TTTTCCCTCTCTC-CTTTTTTCA-3') and TI57M2 (5'-AGCAAAGACAAAGTAGGCTTCC-3'), derived from clone pTI-MD57, produced a product of 118 bp which was unique to T. indica.

REFERENCES:
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Genbank Accession No. X75409.
Ferreira et al., Phytopathology 85(10): Abstract No. 490, p. 1175 (1995).
Smith et al., Phytopathology 84(10): Abstract No. 679, p. 1152 (1994).
Bej et al. Critical Reviews in Biochemnistry and Molecular Biology 26(3/4) :301-334 (1991).
Steffan et al., Annual Reviews in Microbiology 45 : 137-161(1991).

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