Method for increasing the sensitivity of nucleic acid hybridizat

Details Method for increasing the sensitivity of nucleic acid hybridizat Method for increasing the sensitivity of nucleic acid hybridizat

1985-07-25

1989-04-25

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435803, 436501, 536 27, 935 2, 935 8, 935 9, 935 10, 935 78, C12Q 168, G01N 33566

Patent

active

048247766

ABSTRACT:
An improvement in the sensitivity of hybridization assays for detecting polynucleotide target sequences is provided by selective dehybridization of the bound portion of the probe, separation of the dehybridized portion from the support and the residual sample, and concentrating the separated probe before detecting its presence by means of its reporter group. The concentration step can be carried out by adsorption of the dehybridized probe onto a basic ion exchange material. A displacer sequence having greater binding affinity for the target polynucleotide sequence may be used to dehybridize the bound probe portion for subsequent concentration.

REFERENCES:
patent: 4430496 (1984-02-01), Abbott
patent: 4486539 (1984-12-01), Ranki et al.
Green et al, "Reassociation Rate Limited Displacement of DNA Strands", Nucleic Acids Res. 9(8), 1905-1918 (1981).

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