Sugar-regulatory sequences in alpha-amylase genes

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 236, 800287, 8003202, C12N 1511, C12N 1529, C12N 1582

Patent

active

060489731

ABSTRACT:
A chimeric plant promoter which is responsive to sugar depletion or deprivation is disclosed. The promoter includes a promoter element and, carried in the element, a heterologous sequence from the rice Amy3D that is responsive to sugar depletion. Also disclosed is a plant transfecting vector containing the sequence, and plant cell transfected with the vector.

REFERENCES:
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Huang, N., et al., "Classification and characterization of the rice .alpha.-amylase multigene family", Plant Molecular Biology 14 (1990) 655-668.
Huang, N., et al., Structural organization and differential expression of rice .alpha.-amylase genes, Nucleic Acids Research 18, No. 23 (1990) 7007-7014.
Chan, Ming-Tsair, et al., "Novel Gene Espression System for Plant Cells Based on Induction of .alpha.-Amylase Promoter by Carbohydrate Starvation," The Journal of Biological Chemistry 269(26):17635-17641 (1994).
Huang, Ning, et al., "Metabolic regulation of .alpha.-amylase gene expression in transgenic cell cultures of rice," Plant Mol. Biology 23: 737-747 (1993).
Hwang, Y.-S., et al., "Three cis-elements required for rice .alpha.-amylase Amy3D expression during sugar starvation," Plant Mol. Biology 36: 331-341 (1998).
Mitsunaga, Shin-ichiro, et al., "Sequence-specific interactions of a nuclear protein factor with the promoter region of a rice gene for .alpha.-amylase, RAmy3D," Nuc. Acids Res. 22(11):1948-1953 (1994).
Thomas, Bruce R., et al., "Gene Regulation and Protein Sectretion from Plant Cell Cultures: the Rice .alpha.-amylase System," Advances in Plant Biotechnology 4:37-55 (1994).

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