Use of cna, fnba, fnbb, and hlb, gene probes for the strain-spec

Details Use of cna, fnba, fnbb, and hlb, gene probes for the strain-spec Use of cna, fnba, fnbb, and hlb, gene probes for the strain-spec

1996-06-20

1998-08-04

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435810, 536 2432, 935 6, 935 77, 935 78, C12Q 170, C07H 2104

Patent

active

057891710

ABSTRACT:
The present invention provides a method of clinically differentiating the presence of Staphylococcus aureus in isolated samples, comprising the step of: analyzing the hybridization profile observed in Southern blots utilizing DNA probes for genes selected from the group consisting of the cna gene, the fnbA gene, the fnbB gene, the hlb gene, the cna-up gene, the pcp gene, the pcp12 gene and the pcp34 gene. Also provided is a kit for clinically differentiating the presence of Staphylococcus aureus in isolated samples, comprising: (1) restriction enzymes to digest genomic DNA contained in said samples; and (2) DNA probes for genes selected from the group consisting of the cna gene, the fnbA gene, the fnbB gene, the hlb gene, the cna-up gene, the pcp gene, the pcp12 gene and the pcp34 gene.

REFERENCES:
Minhas et. al J. Med. Microbiol. 42:96-101 (Feb. 1995).
Monzon-Moreno et. al. J. Med. Microbiol. 35:80-88 (Aug. 1991).

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