Polypeptides having .beta.-fructofuranosidase activity

Details Polypeptides having .beta.-fructofuranosidase activity Polypeptides having .beta.-fructofuranosidase activity

1997-06-06

1999-10-05

Achutamurthy, Ponnathapura

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435193, 536 234, 536 231, C12N 910, C12N 924, C07H 2104, C07H 2102

Patent

active

059622970

ABSTRACT:
Disclosed are a polypeptide having a .beta.-fructofuranosidase activity obtainable by the expression of a microbial gene, a DNA encoding the polypeptide, a transformant obtained by introducing the DNA into an appropriate host, a process for producing the polypeptide comprising culturing the transformant to produce the polypeptide and collecting the produced polypeptide from the culture, and a method for fructofuranosyl transfer comprising a step of reacting a fructofuranosyl donor with a fructofuranosyl acceptor in the presence of the polypeptide.

REFERENCES:
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Akamatsu, Takashi et al., "Transformation of Bacillus Protoplasts by Plasmid pTP4 DNA." Agric. Biol. Chem., vol. 46, No. 6, pp. 1617-1621 (1982).
Schaeffer, Pierre et al., "Fusion of bacterial protoplasts." Proc. Natl. Acad. Sci. USA, col. 73, No. 6, pp. 2151-2155 (1976).
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