Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1998-12-11
1999-10-05
Stucker, Jeffrey
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 79, 435 792, 436518, 436531, G01N 3353
Patent
active
059622369
ABSTRACT:
Urinary assay for measuring bone resorption, by contacting an unhydrolyzed urine sample with an antibody that binds to free lysyl pyridinoline cross-links, detecting any binding of the antibody in the body fluid sample, and correlating the detected binding to bone resorption in vivo. The free lysyl pyridinoline cross-links are preferably measured as a ratio to the creatinine content in order to provide a urinary index of bone resorption independent of urine volume.
REFERENCES:
patent: 4628027 (1986-12-01), Gay
patent: 4973666 (1990-11-01), Eyre
patent: 5607862 (1997-03-01), Eyre
Eyre, D.R., "Crosslink maturation in bone collagen," In: Veis, A., ed. the Chemistry and Biology of Mineralized Connective Tissues, Elsevier North Holland, Inc., 1981.
Gunja-Smith, Z. and Boucek, R.J., "Collagen cross-linking compounds in human urine," Biochemical Journal, 197:759-762 (1981).
Barber, M., et al., "The structure(s) of pyridinoline(s)," Biochemical and Biophysical Research Communications, 109(3):1041-1046, 1982.
Ogawa, T., et al., "A novel fluor in insoluble collagen: a crosslinking moiety in collagen molecule," Biochemical and Biophysical Research Communications, 104(4:1252-1257, 1982.
Eyre, D.R., "Collagen cross-linking," In: Akeson, W.H. et al., eds., AAOS: Symposium on Heritable Disorders of Connective Tissue, St. Louis: C.V. Mosby, 1982, pp. 43-58.
Robins, S.P., "An enzyme-linked immunoassay for the collagen cross-link pyridinoline," Biochemical Journal, 207:617-620 (1982).
Fujimoto, D., et al., "Analysis of Pyridinoline, a Cross-Linking Compound of Collagen Fibers, in Human Urine," J. Biochem., 94:1133-1136 (1983).
Eyre, D.R., et al., "Cross-Linking in Collagen and Elastin," Ann. Rev. Biochem., 53:717-748 (1984).
Eyre, D.R., et al., "Quantitation of Hydroxypyridinium Crosslinks in Collagen by High-Performance Liquid Chromatography," Analytical Biochemistry, 137:380-388 (1984).
Wu, et al., "Identification of Hydroxypyridinium Cross-Linking Sites in Type II Collagen of Bovine Articular Cartilage," Biochemistry 23:1850-1857 (1984).
Wu, et al., "Cartilage type IX collagen is cross-linked by hydroxypyridinium residues," Biochemical and Biophysical Research Communications 123(3):1033-1039 (1984).
Robins, S.R., et al., "Measurement of the cross linking compound, pyridinoline, in urine as an index of collagen degradation in joint disease," Annals of the Rheumatic Diseases, 45:969-973 (1986).
Eyre, D., "Collagen Cross-Linking Amino Acids," In: Methods in Enzymology, vol. 144, pp. 115-139, Academic Press, Inc., 1987.
Eyre, D.R., "Collagen Stability Through Covalent Crosslinking," In: Pearson, et al., eds. Advances in Meat Research, vol. 4, Collagen as a Food. New York: Van Nostrand Reinhold, 1987.
Robins, S., et al., "Pyridinium crosslinks of bone collagen and their location in peptides isolated from rat femur," Biochemica et Biophysica Acta, 914(3):233-239, 1987.
Robins, S.P., et al., "Measurement of Hydroxypyridinium Crosslinks of Collagen as an Index of Bone Matrix Degradation," Abstract, Lake Garda, Italy (1987).
Macek, J., et al., "Determination of collagen degradation products in human urine in osteoporosis," Z. Rheumatol., 46:237-240, 1987.
Stucker Jeffrey
Washington Research Foundation
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