Process for concentrating and purifying human urinary kallikrein

Details Process for concentrating and purifying human urinary kallikrein Process for concentrating and purifying human urinary kallikrein

1983-06-29

1985-04-09

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435212, 435219, C12N 964, C12N 948, C12N 950

Patent

active

045102481

ABSTRACT:
Human urinary kallikrein is concentrated and purified by the following procedures; (a) Human urine is contacted with chitosan, a high molecular agglutinant obtained from chitin, at a pH from 4.0 to 7.0, thereby kallikrein is adsorbed on the chitosan and (b) kallikrein is eluted from the chitosan with an aqueous alkaline solution having a pH from 8.0 to 12.0.

REFERENCES:
patent: 4393140 (1983-07-01), Schutt
Geiger et al., "Human Urinary Kallikrein", Methods in Enzymology, vol. 80, pp. 466-492 (1981).
Biotechnology and Bioengineering, vol. 20, pp. 87-94 (1978).

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