Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1988-03-28
1990-02-13
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4351723, 4352402, 4352523, 43525233, 435320, 536 27, 935 14, 424 9461, C12N 1500, C12N 922, C12N 120, C07H 1512
Patent
active
049006738
ABSTRACT:
Site-specific mutagenesis of a gene for angiogenin producing DNA sequences encoding mutant proteins having increased angiogenic activity are disclosed. Expression vectors containing these sequences are introduced into host cells and direct the production of the mutant angiogenic proteins with markedly increased angiogenic and ribonucleolytic activity. Replacement of a single amino acid, the aspartic acid at position 116 of human angiogenin, with another amino acid including asparagine, alanine or histidine, yields mutant proteins with 8 to 15 fold increased ribonucleolytic activity toward tRNA and rRNA and 10 to 100 fold increased angiogenic potency in the chorioallantoic membrane assay. The mutant angiogenin proteins of this invention are useful therapeutic compositions to promote the development of a hemovascular network in a mammal or to promote wound healing, in particular, healing of torn or traumatized fibrocartilage material.
REFERENCES:
patent: 4721672 (1988-01-01), Vallee
Harper Jeffrey W.
Vallee Bert L.
Brown Anne
President and Fellows of Harvard College
Wiseman Thomas G.
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