Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1986-02-12
1990-02-13
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
4351722, 435810, 436501, 436548, 436808, 530387, 935 89, 935 95, G01N 3353, G01N 33566, C12N 1500
Patent
active
049006614
ABSTRACT:
This method for immunological determination of amines in biological media consists of: (a) reacting a quinone (in particular, p-benzoquinone) with the amine (for example histamine) present in the test sample; (b) introducing into the medium a monoclonal antibody which specifically recognizes the amine-quinone reaction product formed; and (c) determining one of the forms of the monoclonal antibody present. When the quinone is used in the immobilized state, the monoclonal antibody present is determined in its complexed form with the amine-quinone reaction product (direct type method); when the quinone is used in the free state, the monoclonal antibody is determined in its free form (enzymoimmunometric method or competitive immunoenzymatic method).
REFERENCES:
patent: 4481298 (1984-11-01), Cone, Jr. et al.
Avrameas Stratis
Guesdon Jean-Luc
Mazie Jean-Claude
Centre National de la Recherche Scientifique "CNRS"
Graeter Janelle
Institut Pasteur Paris
Warden Robert J.
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