Separation of hemoglobin A.sub.2 from hemoglobin mixture

Details Separation of hemoglobin A.sub.2 from hemoglobin mixture Separation of hemoglobin A.sub.2 from hemoglobin mixture

1988-12-19

1990-12-25

Therkorn, Ernest G.

Liquid purification or separation

With means to add treating material

Chromatography

210139, 210143, 73 611C, 422 70, B01D 1508

Patent

active

049800582

ABSTRACT:
An ion exchange method for the separation of hemoglobin A.sub.2 from other hemoglobin components normally present in human blood involves the use of three buffer solutions in succession, with specified ionic strengths and pHs. The first has a phosphate buffer at 1 to 20 mM and a pH of 6.5 to 6.9; the second has phosphate buffer at 15 to 55 mM and a pH of 6.4 to 6.8; and the third has phosphate buffer at 60 to 100 mM and a pH of about 6.4 to 6.8. The first is continued until substantially all hemoglobin A.sub.1a and A.sub.1b has eluted from the column, the second until substantially all A.sub.1c, F, and A.sub.0 is eluted, and the third for the elution of A.sub.2.

REFERENCES:
patent: 4108603 (1978-08-01), Regnier
patent: 4159895 (1979-07-01), Sline
patent: 4209372 (1980-06-01), Bluestein
patent: 4209373 (1980-06-01), Bluestein
patent: 4448888 (1984-05-01), Bleile
patent: 4502786 (1985-03-01), Golias
patent: 4604350 (1986-08-01), DeMatteis
patent: 4764279 (1988-08-01), Tayot
DIAMAT Fully Automated Glycosylated Hemoglobin Analyzer System, Instruction Manual, Nov. 1985.

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