Process and composition for the rapid quantitation of small leve

Details Process and composition for the rapid quantitation of small leve Process and composition for the rapid quantitation of small leve

1984-04-06

1986-11-25

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

424 85, 435 17, 435810, 436524, 436525, 436526, 436527, 436528, 436529, 436530, 436531, 436532, 436533, 436534, 436547, 436808, 436811, 436825, 436826, 530387, 530389, 530402, A61K 3900, G01N 3353

Patent

active

046249168

ABSTRACT:
A process for conducting fast, rapid two site, two step immunoradiometric tests for CK-MB.
In a first series of steps, a mobile particulate solid-phase having an isoenzyme selected from the group of CK-BB and CK-MM immobilying bound on its surface is suspended in a matrix of pooled match human serum. The mixture is incubated, diluted, and centrifuged.
In a second series of steps, the resulting solid is incubated with a radio labeled antibody that is specific to the isoenzyme not used in the first series of steps. Counting the radioactivity gives the amount of CK-MB in the patients serum.

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Biagini et al., from Radioimmunoassay of Drugs and Hormones in Cardiovascular Medicine, Albertini et al., Eds., Elsevier/North Holland Biomedical Press, Amsterdam, 1979, pp. 85-92.

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