Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1999-01-11
2000-08-08
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 6, 435 71, 435 72, 435 795, 435334, 435355, 435810, 435968, 436501, 436513, 436521, 436536, 436 94, 436800, 5303887, 5303871, 935 76, 935 77, C12Q 168, G01N 3353, G01N 33566, G01N 33563, C07K 1600
Patent
active
061000388
ABSTRACT:
A single laser flow cytometric method for the enumeration of micronuclei in erythrocyte populations, wherein a sample of peripheral blood or bone marrow is obtained and the cell populations in the sample are fixed. Reticulocytes in the fixed samples are treated simultaneously with RNAse and with a fluorescent labeled antibody having binding specificity for a surface marker for erythroblasts/reticulocytes. The erythrocyte populations are then stained with a nucleic acid stain which stains DNA representing micronuclei, if present. The stained and/or labeled erythrocyte populations are then exposed to a laser beam of appropriate excitation wavelength for both the nucleic acid staining dye and the fluorescent label to produce fluorescent emission. The fluorescent emission and light scatter produced by the erythrocyte populations are detected by the flow cytometer from which is calculated the number of specific erythrocyte populations in said sample.
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Dertinger Stephen D.
Tometsko Kenneth R.
Torous Dorothea K.
Gabel Gailene R.
Housel James C.
Litron Laboratories Limited
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