Autoradiographic process

Details Autoradiographic process Autoradiographic process

1989-07-06

1993-11-09

Lovering, Richard D.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436 57, 436 94, 935 77, 250581, 2504842, C12Q 168, G03C 516, G01N 33566

Patent

active

052601900

ABSTRACT:
An autoradiographic process for obtaining information on one or two dimensional location of radioactively labeled substances contained in a sample selected from the group consisting of tissue of an organism and a medium containing tissue of an organism and/or substances such as DNA originating from an organism,
which comprises:
placing said sample and a stimulable phosphor sheet having a phosphor layer comprising a stimulable phosphor dispersed in a binder together in layers for a certain period to cause said phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances in said sample;
scanning said phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet as stimulated emission; and
detecting the stimulated emission to obtain locational information on the radioactively labeled substances in the sample.

REFERENCES:
patent: 4239968 (1980-12-01), Kotera et al.
patent: 4389670 (1983-06-01), Davidson et al.
patent: 4617468 (1986-10-01), Shiraishi et al.
patent: 4734581 (1988-03-01), Hashiue
patent: 4803359 (1989-02-01), Hosoi et al.
patent: 4852050 (1989-07-01), Shiraishi et al.
patent: 4865967 (1989-09-01), Shiraishi et al.
European Search Report, Application No. EP 83 11 0984.
Laskey, R. A. et al., FEBS Letters, vol. 82, No. 2, pp. 314-316 (1977).
Swanstrom et al., Analytical Biochemistry, vol. 86, pp. 184-192 (1978).

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