Method for mammalian cell transfection

Details Method for mammalian cell transfection Method for mammalian cell transfection

1994-04-25

1996-12-31

Stanton, Brian R.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 6, 435 721, 4352401, 4352402, 43524021, 4352403, 435948, 935 52, 935 55, C12N 1500, C12N 100, C12N 113, C12Q 168

Patent

active

055893686

ABSTRACT:
The present invention relates to a process for transfecting a mammalian cell culture. The process includes incubating a cell culture in the presence of a transfection medium that includes a serum that is of a different type from the serum used in the normal growth medium used to grow the cell culture. It is preferred that the normal growth medium include fetal bovine serum and the transfection medium include a serum such as human, calf, horse, lamb, or pig. The transfection medium may further include an hydryoxylated sterol such as 25-hydroxycholesterol.

REFERENCES:
patent: 5330904 (1994-07-01), Spear et al.
Ness et al., Eds., 1989, Analysis of Sterols and Other Biologically Significant Steroids; Academic Press, Inc., San Diego, pp. 1-119.
Chen et al., 1987, Molecular and Cellular Biology, 7(8):2745-2752.
Freshney, 1987, Culture of Animal Cells: A Manual of Basic Techniques; Alan R. Liss, Inc., N.Y. p. 37-84, 139 and 216-217.

Affiliated with

Also associated with

Rating

Method for mammalian cell transfection does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for mammalian cell transfection, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for mammalian cell transfection will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1141460