Origin of replication complex genes and methods of using the sam

Details Origin of replication complex genes and methods of using the sam Origin of replication complex genes and methods of using the sam

1995-06-07

1996-12-31

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 692, 4352402, 4352551, 935 59, 935 70, 935 77, 53038821, C12Q 168, C12N 1500, C12N 115, C07K 1439

Patent

active

055893414

ABSTRACT:
Origin of Replication Complex (ORC) genes, nucleic acids which encode ORC proteins and hybridization reagents, probes and primers capable of hybridizing with ORC genes and methods for screening chemical libraries for lead compounds for pharmacological agents useful in the diagnosis or treatment of disease associated undesirable cell growth are provided. An exemplary screen involves forming a mixture comprising a recombinant ORC protein, a natural intracellular ORC protein binding target, and a candidate pharmacological agent; incubating the mixture under conditions whereby, but for the presence of said candidate pharmacological agent, said ORC protein selectively binds said binding target; and detecting the presence or absence of specific binding of said ORC protein to said binding target.

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