Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Patent
1982-09-28
1995-11-21
Martinell, James
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
435 6951, C07K 1452, C12P 2100
Patent
active
054686091
ABSTRACT:
The present invention relates to a process to isolate genetic material (DNA) containing the nucleotide sequence coding for interferon in human fibroblastic cells which comprises cultivating cells producing interferon when exposed to an inducer of interferon, exposing same to such inducer, extracting messenger RNA from said induced cells, purifying the interferon messenger RNA, transcribing the messenger RNA into DNA and cloning the DNA in a suitable vector. Preferred cells are human diploid foreskin cells. The invention further relates to a process for engineering a bacterial strain to produce interferon polypeptide which comprises introducing a cloned interferon DNA into a suitable vector-carrier. A preferred vector-carrier is E. coli. The invention also relates to the mRNA of human interferon in highly purified form, to the mRNA of human interferon in .beta.1 highly purified form, to the mRNA of human interferon in .beta.2 highly purified form, to the DNA coding for a polypeptide having interferon activity, insertable in a vector, such as plasmid pBR322, and also to human interferon .beta.1 in highly purified form, and human interferon .beta.2 in highly purified form.
REFERENCES:
patent: 4262090 (1981-04-01), Colby et al.
patent: 4289689 (1981-09-01), Friesen et al.
patent: 4359389 (1982-11-01), Heine
Kwan et al.: Proc. Natl. Acad. Sci. USA 74, 178 (1977).
Goeddel et al.: Proc. Natl. Acad. Sci. USA 76, 106 (1979).
Vilcek et al.: in Human Interferon, Production and Clinical Use, Stinebring et al. (ed.), Plenum Press, New York, 1978, pp. 101-118.
Edy et al.: in Human Interfeon, Production and Clinical Use, Stinebring et al. (ed.), Plenum Press, New York, 1978, pp. 55-60.
St. John et al., Cell 16: 443 (1979).
Williams et al., J. Mol. Biol. 129: 19 (1979).
Weissenbach et al., Proc. Natl. Acad. Sci. USA 77: 7152 (1980).
Gene Expression -2, Eucaryotic Chromosomes, Lewin, 1974, John Wiley & Sons, New York, N.Y., pp. 148-151.
Burke, in The Interferon System: A Current Review to 1978, Baron et al. (ed.), Texas Reports on Biology and Medicine, University of Texas Medical Branch, Galveston, Tex., vol. 35, pp. 11-14 (1977).
Handbook of Biochemistry, Selected Data for Molecular Biology, 2nd Edition, Sober et al. (ed.), Chemical Rubber Co., Cleveland, Ohio (1970), pp. H-104 and H-113 (compiled by H. Shapiro).
Revel Michel
Tiollais Pierre
Martinell James
Yeda Research & Development Co. Ltd.
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