Method of purifying alcohol dehydrogenase and affinity resin the

Details Method of purifying alcohol dehydrogenase and affinity resin the Method of purifying alcohol dehydrogenase and affinity resin the

1978-05-10

1978-12-26

Hamrock, William F.

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Polymers from only ethylenic monomers or processes of...

195 66R, 526 50, 536112, C08F 830, C08F 832

Patent

active

041317270

ABSTRACT:
An affinity resin containing pyrazole immobilized on a solid insoluble support by a coupling arm bonded at the 4-position having a length of at least 12 Angstrom units is used to purify and fractionate crude alcohol dehydrogenase extract into a pyrazole-binding fraction and a pyrazole-insensitive fraction containing the anodic band by bringing the resin into contact with an aqueous solution of a binary complex of alcohol dehydrogenase and NAD to bind the binary complex, then eluting the complex. The bound fraction can be further fractionated into isoenzymes during elution by adjusting the alcohol gradient of the eluant, and the anodic band can be separated from the remainder of the solution, which does not bind to pyrazole, by salting out and chromatography on agarose-AMP resin.

REFERENCES:
patent: 3912595 (1975-10-01), Philipp et al.
patent: 3920631 (1975-11-01), Molteni et al.
patent: 3926730 (1975-12-01), Huper et al.
patent: 3951744 (1976-04-01), Rodev et al.
patent: 3970603 (1976-07-01), Gray
patent: 4030997 (1977-06-01), Fujii et al.

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