Optics: measuring and testing – By particle light scattering – With photocell detection
Patent
1995-12-20
1999-08-10
Turner, Samuel A.
Optics: measuring and testing
By particle light scattering
With photocell detection
25033902, 2504612, G01B 902
Patent
active
059367318
ABSTRACT:
A fluorescent in situ hybridization method comprising the steps of (a) providing a cell nuclei having chromosomes hybridized with at least one nucleic acid probe including at least one nucleic acid molecule labeled with at least one fluorophore; (b) viewing the cell nuclei through a fluorescence microscope optically connected to an imaging spectrometer for obtaining a spectrum of each pixel of the cell nuclei by (i) collecting incident collimated light simultaneously from all pixels of the cell nuclei; (ii) passing the incident collimated light through an interferometer system so that the light is first split into two coherent beams and then recombine to interfere and form an exiting light beam; (iii) focusing the exiting light beam on a detector having an array of detector elements, so that at each instant each of the elements is the image of one and always the same pixel for the entire duration of the measurement and so that each of the elements produces a signal which is a particular linear combination of light intensity emitted by the pixel at different wavelengths; (iv) rotating or translating one or more of the elements of the interferometer, so that the optical path difference is scanned simultaneously for all the pixels of the cell nuclei; and (v) recording signals of each of the detector elements as function of time using a recording device to form a first spectral cube of data; and (c) interpreting the first spectral cube of data using a mathematical algorithm.
REFERENCES:
patent: 4930516 (1990-06-01), Alfano et al.
patent: 4976542 (1990-12-01), Smith
patent: 5377003 (1994-12-01), Lewis et al.
patent: 5432865 (1995-07-01), Kasdan et al.
patent: 5436978 (1995-07-01), Kasdan et al.
patent: 5539517 (1996-07-01), Cabib
Andersson-Engels et al, "Multicolor Fluorescence Imaging System for Tissue Diagnostics", SPIE vol. 1205 Bioimaging and Two Dimensional Spectroscopy, pp. 179-189 (1990).
Chamberlain, J. "the Principles of Interferomertric Spectroscopy", John Wiley & Sons pp. 16-18, 263.
Wied, G. et al, "Computer Discrimination in Ectocervical Cells". Anal. And Quant Cytology, No. 3, vol. 3, Sep. 1981.
International Remote Imaging Systems (IRIS) News Release, Jul. 26, 1995.
Ried, T., "Fluoreszenz In Situ Hybridisierung in der Genetischen Diagnostik", 1994.
Wellman, J., "Imaging Spectrometers for Terresrial and Planetary Remote Sensing: A Progress Report", SPIE, vol. 750 Infrafred Systems and Components pp. 140-152 (1987).
Dozier, J., "HIRIS--The High Resolution Imaging Spectrometer", SPIE, vol. 924, pp. 23-30 (1988).
Valisa, P. et al, "Comparison and Applications to in-vivo Biology of Two Different Methods of Spectral Imaging: Confocal Scanning-Full Spectrum and Global Illumination Spectral Band-Pass Imaging", SPIE, vol. 2627, pp. 156-164, May 1995.
Maymon, P. et al, "Optical System Design Alternatives for the Moderate-Resolution Imaging Spectrometer-Tilt (MODIS-T) for the Earth Observing System (EOS)", SPIE, vol. 924, pp. 10-22 (1988).
Buckwald Robert A.
Cabib Dario
Garini Yuval
Katzir Nir
Lavi Moshe
Applied Spectral Imaging Ltd.
Friedman Mark M.
Turner Samuel A.
LandOfFree
Method for simultaneous detection of multiple fluorophores for i does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for simultaneous detection of multiple fluorophores for i, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for simultaneous detection of multiple fluorophores for i will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1125520