Method of effecting immunoassay by using affinity chromatography

Details Method of effecting immunoassay by using affinity chromatography Method of effecting immunoassay by using affinity chromatography

1987-03-31

1990-06-26

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436161, 436541, 364497, G01N 33543

Patent

active

049372009

ABSTRACT:
A method of effecting an immunoassay using affinity chromatography which a sample is injected into a reusable column charged with antibody applied on a solid support, an eluent is injected into the column to elute antigen bound with the antibody on the solid support, and an amount of eluted antigen is measured. In order to mitigate the influence of carry-overs of successive samples, blank values are corrected such that they include the carry-overs of previously measured samples, while elution time periods are kept constant. In one embodiment, the elution time periods are corrected such that blank values for respective samples are made constant.

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de Alwis, Anal. Chem. vol. 57, 1985, pp. 2754-2756.
H. Oster, Prozess Chematographie, Frankfort 1973, p. 263.

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