Process for micro biological production of proteins

Details Process for micro biological production of proteins Process for micro biological production of proteins

1997-05-29

1999-08-10

Mertz, Prema

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435212, 435219, 435 712, 435325, 4352523, 4353201, 435471, 530407, C12N 516, C12N 1563

Patent

active

059358157

ABSTRACT:
The invention is based on the finding that furin belongs to a family of endoproteolytically active enzymes and relates to a process in the in vitro cleavage of a protein by treating the protein in the presence of Ca.sup.2+ ions with furin, or an endoproteolytically active fragment, derivative or fusion protein of furin. The invention can be used for the (micro) biological production of a protein by culturing genetically engineered cells expressing a pro-form of the protein as well as furin and isolating the protein formed. The invention also relates to a pharmaceutical composition comprising one or more pharmaceutically acceptable carriers, diluents or adjuvants, as well as an endoproteolytically active amount of furin, or a fragment or derivative of furin having an endoproteolytic activity.

REFERENCES:
Fuller et al. Intracellular targetting and structural conservation of a prohormone-processing endoprotease. Science. vol. 246, pp. 482-486., Oct. 27, 1989.
Roebroek et al. Evolutionary conserved close linkage of the c-fes/fps proto-oncogene and genetic sequences encodinga receptor-like protein. The EMBO Journal. vol. 5, No. 9, pp. 2197-2202., 1986.
Thomas et al. Yeast KEX2 endopeptidase correctly cleaves a neuroendocrine prohormone in mammalian cells. Science. vol. 241, pp. 226-230., Jul. 8, 1988.
Wise et al. Expression of a human proprotein processing enzyme: correct cleavage of the von Willebrand factor precursor at a paired basic amino acid site. Proceedings of the National Academy of Sciences, USA. vol. 87, pp. 9378-9382., Dec. 1990.

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