Plant-derived enzyme and DNA sequences and uses thereof

Details Plant-derived enzyme and DNA sequences and uses thereof Plant-derived enzyme and DNA sequences and uses thereof

1996-06-17

1999-02-02

Robinson, Douglas W.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

4351723, 435193, 4353201, 435419, 536 232, 536 236, 800250, 47 58, A01H 500, A01H 510, C12N 910, C12N 1529, C12N 1552, C12N 1585

Patent

active

058667920

ABSTRACT:
The chemically-inducible 27 kD subunit of the enzyme glutathione-S-transferase, isoform II (GST-II-27) and sequences encoding it are provided. In particular, a genomic DNA sequence encoding the gene promoter for the GST-II-27 subunit is provided. Then linked to an exogenous gene and introduced into a plant by transformation, and GST-II-27 promoter provides a means for the external regulation of expression of that exogenous gene. Transformation with DNA encoding glutathione-S-transferase polypeptides produces herbicide resistance transgenic plants.

REFERENCES:
patent: 5073677 (1991-12-01), Helmer et al.
Biochemistry, vol., No. 5, 1983, pp. 1068-1072, Mozer, T. J., et al, "Purification and characterization of com glutathione S-transferase" see the whole document.
WOA 9,008830, Aug. 9, 1990, see the whole document.
Plant Molecular Biology, vol. 6, No. 4, 1986, pp. 203-211, Shah et al, Structural analysis of glutathione-S-transferase involved in herbicide detoxification see the whole document.
WO, A, 9 008 830, Aug. 9, 1990, see p. 1-p. 7, see p. 18-p. 25, figures 1,2.
Biotechnology vol. 3, Jul. 1985, pp. 629-635, Fraley, et al: "The SEV system: a new disarmed Ti plasmid vector system for plant transformation", see p. 632, right column, paragraph 1.
Gene, vol. 76, 1989, pp. 153-160, Wosnick et al: "Total chemical synthesis and expression in Eschericha coli of a maze glutathione-transferase (GST) gene" see the whole document.
Nucleic Acids Research, vol. 14, No. 18, 1986, pp. 7227-7235 Moore et al: "Cloning and expression of a cDNA encoding a maize glutathione-S-transferase gene in E. coli" see page 7227 introduction para. 2.
Physiol. Plant. vol. 77, No. 3, 1989, pp. 465-471 Timmerman, "Molecular characterization of com glutathione S-transferase isozymes involved in herbicide detoxification".
Nucleic Acids Research, vol. 16, No. 2, 1988, pp. 425-438, Grove et al; "Characterization and heterospecific expression of cDNA clones of genes in the maize GSH S-transferase multigene family" see the whole document.
J. Cell. Biochem. Suppl., Meeting Apr. 10-16, 1992, vol. 16F, 1992, p. 232, Greenland et al: "Cloning and analysis of a cDNA clone encoding a glutathione-S-transferase subunit from maize which is induced by treatment with herbicide safeners", See Abstract Y412.

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