Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Animal or plant cell
Patent
1995-04-07
1998-08-18
Naff, David M.
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Animal or plant cell
264 4, 264 43, 264 432, 424424, 424572, 4284022, 42840224, 435178, 435182, 435325, 435363, 435366, 435382, A61K 3512, C12N 1110, B01J 1320
Patent
active
057955700
ABSTRACT:
A core material such as animal tissue or cells is contained within a semipermeable vessel which may be a microcapsule, hollow fiber or plastic membrane having a semipermeable wall by a method that prevents the core material from incorporation into the wall of the vessel. This is accomplished by suspending the core material in a solution of polysaccharide gum such as an alkali metal alginate in an amount between about 0.2% and about 0.5%, removing and washing the core material to remove all but a thin layer of polysaccharide gum, and gelling the polysaccharide gum with multivalent cations or other means to form a pretreated core material. The pretreated core material is contained within a semipermeable vessel such as by suspending the pretreated core material in a solution of alkali metal alginate, forming the suspension into droplets, gelling the droplets to form temporary shape-retaining capsules and treating the capsules with a polymeric material having groups that react with and crosslink acid groups of the capsules to form a permanent semipermeable membrane around the capsules. A second permanent semipermeable membrane may be formed around the capsules to form double-walled microcapsules by further treating the capsules with the polymeric material. The semipermeable vessel may be impermeable to immune factors. Cells or tissue can be transplanted from a donor to a subject such as by using pancreatic islet tissue or cells as the core material of the double-walled microcapsules and transplanting the microcapsules by intraperitoneal injection into a diabetic subject.
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Ayres-Price Jennifer
Weber Collin J.
Emory University
Naff David M.
White John P.
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