Separation of interleukin-2 from phytohemagglutinin by dye matri

Details Separation of interleukin-2 from phytohemagglutinin by dye matri Separation of interleukin-2 from phytohemagglutinin by dye matri

1982-12-14

1985-04-02

Padgett, Ben R.

Chemistry: analytical and immunological testing

Involving producing or treating antigen or hapten

436807, 210660, 210672, 210927, 260112R, 424 88, 514 2, G01N 3108, A61K 3702, B01D 1508

Patent

active

045088330

ABSTRACT:
Crude interleukin-2 extract is subjected to group-selective dye-ligand absorption chromatography in one or more stages of purification with a matrix-gel medium consisting of a Blue A ligand or variant thereof or of a Green A ligand in a concentration of approximately 1.5 to 3.0 mg/ml of expanded matrix at a pH of approximately 6.8 to 8.5, a temperature of approximately 4.degree. to 40.degree. C., and a flowthrough rate of approximately 10 to 100 ml/h, employing an eluent. Either PHA-free or extremely pure interleukin-2 is obtained, depending on the overall number of purification stages.

REFERENCES:
patent: 4355017 (1982-06-01), Miles et al.
patent: 4390623 (1983-06-01), Fabricus et al.
patent: 4404188 (1983-09-01), Donahoe et al.
patent: 4406830 (1983-09-01), Fabricius et al.
Dye-Ligand Chromatography, Amicon Corporation, Publication 512, Lexington, Mass. (1980), pp. C2-C37.

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