Ellipsometrically measuring rate of optical change in immunoassa

Details Ellipsometrically measuring rate of optical change in immunoassa Ellipsometrically measuring rate of optical change in immunoassa

1982-06-18

1985-04-02

Marantz, Sidney

Chemistry: analytical and immunological testing

Involving kinetic measurement of antigen-antibody reaction

356246, 356364, 436527, 436805, G01N 3354, G01N 2117, G01N 2121

Patent

active

045088322

ABSTRACT:
A layer of bioactive molecules is coated on a dielectric substrate and is contacted with a solution to be analyzed containing a complex conjugate of said molecule. The rate of complexion of said conjugate moiety with the layer which is a function of its concentration in the analyte is measured by optical means and correlated with corresponding rates obtained from standard reference measurements, thus providing data for determining the unknown concentration of said conjugate moiety in the solution to be analyzed.

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W. E. J. Neal et al., Journal of Physics & Scientific Instruments, 6, 409-416 (1973).
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Rothen, A. et al., Helvetica Chimica Acta., 54(4), 1208-1216 (1971).

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