Method and kit for pregnancy detection

Details Method and kit for pregnancy detection Method and kit for pregnancy detection

1982-08-18

1985-04-02

Marantz, Sidney

Chemistry: analytical and immunological testing

Immunochemical pregnancy determination

422 61, 436 65, 436519, 436808, 436814, 436818, 436827, G01N 3354, G01N 3358, G01N 3376

Patent

active

045088292

ABSTRACT:
Method and kit for pregnancy detection adapted for self-performance. Urine to be tested is contacted with a lectin substrate being lectin bound to a solid support and capable of binding HCG. After separation of the lectin substrate from the urine the substrate is contacted with a liquid color reagent comprising a colored carrier material and anti-HCG antibodies bound thereto. If after separation of the colored reagent from the substrate the latter remains colored, the subject is pregnant, while lack of color indicates non-pregnancy. A preferred color reagent comprises killed and stained Staphylococci bacteria. There is also provided a kit for self-performance of the method comprising at least one column packed with a lectin substrate and adapted for the controlled passage of liquid therethrough, and a color reagent.

REFERENCES:
patent: 4105598 (1978-08-01), Yen et al.
patent: 4190628 (1980-02-01), Sears
patent: 4289747 (1981-09-01), Chu
patent: 4302536 (1981-11-01), Longenecker
patent: 4371515 (1983-02-01), Chu
patent: 4419453 (1983-12-01), Dorman
P. G. Natali et al., Jour. of Immunological Methods, 25, 255-264, (1979).
Koyama et al., Folia Endocrinol. Jap. 55, 1979, pp. 133-140, (Abstract in English).
Braciale et al., Journal of Immunological Methods, 43, (1981), pp. 241-250.
Pompecki et al., Cancer Research, 41 (1), (1981), pp. 1905-1909.
Guesdon et al., Journal of Immunological Methods, 39, (1980), pp. 1-13.

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