Gene amplification in eukaryotic cells

Details Gene amplification in eukaryotic cells Gene amplification in eukaryotic cells

1985-04-12

1987-04-07

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 68, 435 70, 4351723, 435240, 435317, 536 27, 935 16, 935 34, 935 36, 935 52, 935 70, 935 71, 935 79, 935 82, C12P 1934, C12P 2100, C12P 2102, C12N 1500, C12N 500, C12N 100, C07H 2104

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046561340

ABSTRACT:
Method and compositions are provided for gene amplification. A DNA unit is prepared capable of replication in a eukaryotic host having tandem genes, where the first gene is capable of complementing an auxotrophic host. The auxotrophic host is transformed with the DNA unit under selective conditions requiring enhanced expression of the first gene. Hosts are then selected for amplification of the first and second genes.

REFERENCES:
Schimke, "Gene Amplification and Drug Resistance", Scientific American 243:60 (Nov. 1980).
O'Hare et al., "Transformation of Mouse Fibroblasts to Methotrexate Resistance by a Recombinant Plasmid Expressing a Prokaryotic Dihydrofolate Reductase", Proc. Natl. Acad. Sci. USA 78: 1527 (1981).
Hamer et al., "Expression of the Chromosomal Mouse Beta Major-Blobin Gene Cloned in SV40", Nature 281: 35 (1979).
Ringold et al., "Co-14 Expression and Amplification of Dihydrofolate Reductase cDNA and the Escherichia coli XGPRT Gene in Chinese Hamster Ovary Cells", J. Molec. Appl. Genet. 1(3): 165 (1981).
Wigler et al., Proc. Natl. Acad. Sci. USA (1980) 77:3567-3570.
Lee et al., Nature (1981) 294:228-232.
Nunberg et al., Proc. Natl. Acad. Sci. USA (1978) 75:5553-5556.
Wahl et al., J. Biol. Chem. (1979) 254:8679-8689.

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