Nif promoter of fast-growing rhizobium japonicum

Details Nif promoter of fast-growing rhizobium japonicum Nif promoter of fast-growing rhizobium japonicum

1985-08-07

1989-02-07

Warren, Charles F.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 68, 435320, 4352522, 43525233, 536 27, 935 29, 935 30, 935 41, 935 56, 935 64, 935 67, 935 72, C12N 1500, C12N 120, C12P 2100, C07H 1512

Patent

active

048031658

ABSTRACT:
The promoter of the nifH gene of the fast-growing Rhizobium japonicum strain USDA 191, has been cloned. Over 4.2 kilobase pairs (kbp) of DNA were sequences (FIG. 1). Sequences encoding nifH and the 5'-end of nifD were identified, as were sequences involved in promoting operon transcription and a nifH ribosome binding site. Use of the nifH promoter to drive transcription in Rhizobium of heterologous structural genes is taught. Useful sequences and plasmids are also disclosed.

REFERENCES:
patent: 4448885 (1984-05-01), Schuepf et al.
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Drummond et al. (1983), "Positive Control . . . of the nifLA Promoter . . . ", Nature 301: 302-307.
Sundaresan et al (1983), "Activation of . . . Nitiogenase Promoters . . . ", DNAS 80: 4030-34.
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