Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Albumin
Patent
1993-06-15
1995-10-03
Russel, Jeffrey E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Albumin
530386, 530414, 530833, C07K 134, C07K 1447, C07K 1476
Patent
active
054553318
ABSTRACT:
Whey protein fractions, especially alpha-lactalbumin and beta-lactoglobulin, are produced by a process which comprises the steps of: (a) treating the whey to achieve a reduction in the specific gravity and ionic strength of the whey to levels which should not be less than 25% of their original values; (b) adjusting the pH of the whey to a value in the range 3.80 to 5.50 by the addition of acid; the above steps being carried out in any order; (c) heating the pH-adjusted whey to a temperature in the range 55.degree.-70.degree. C., and maintaining the whey at that temperature for a period greater than 30 seconds and sufficient to permit aggregation of a portion of the protein content of the whey; (d) cooling the whey to a temperature less than 55.degree. C., and maintaining the whey at that temperature for a period of time sufficient to permit flocculation of the aggregated protein; (e) separating the aggregated protein containing alpha-lactalbumin from the mother liquor; and (f) optionally, recovering beta-lactoglobulin and/or other soluble proteins from the mother liquor.
REFERENCES:
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patent: 4485040 (1984-11-01), Roger et al.
patent: 4782138 (1988-11-01), Rialland et al.
Harwalkar, Milchwissenschaft vol. 41 (4) pp. 206-209 (1986).
Amundson et al., J. Food Process Preserv. vol. 6 (2) pp. 55-72 (1982).
Pearce, R. J., "Fractionation of Whey Proteins" Australian Journal of Dairy Technology, vol. 2298, No. 42, 75-78 (1987).
Pearce, R. J., "Thermal Separation of .beta.-Lactoglobulin and .alpha.-Lactalbumin in Bovine Cheddar Cheese Whey", Australian Journal of Dairy Technology, vol. 38, 144-148 (Dec. 1983).
Patent Abstracts of Japan, JP-61-268138, C-417, p. 142, Nov. 27, 1986.
Commonwealth Scientific and Industrial Research Organisation
Russel Jeffrey E.
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