Immunoassay for cell proteins

Details Immunoassay for cell proteins Immunoassay for cell proteins

1992-01-21

1992-09-22

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 721, 435 72, 436501, 5303871, 5303891, 53038825, 530413, 935102, 935108, G01N 3353

Patent

active

051496275

ABSTRACT:
An immunoassay for cellular proteins which may be present as contaminants in a product purified from mammalian cell culture. Since mammalian cell culture requires the use of protein-containing media, the assay must be specific in recognizing cellular proteins but not media proteins. This is accomplished by selective adsorption of an antiserum to cellular proteins against media proteins. Quantification of the assay may be improved through the use of a purified antigen, namely fibronectin, which is known to be highly immunogenic.

REFERENCES:
Eaton et al., (1987) J. Biol. Chem., vol. 262, No. 7, pp. 3285-3290.
Goding, Monoclonal Antibodies, Principles and Practice, Academic Press, Inc., 1983, Chapter 8, pp. 250-261.

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