Process for the production of hetero-bispecific antibodies

Details Process for the production of hetero-bispecific antibodies Process for the production of hetero-bispecific antibodies

1990-03-22

2000-05-09

Lacey, David L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 43524027, 4353201, 435972, 536 2353, C07H 1512, C12P 2106, C12N 1500, C12N 500

Patent

active

060602856

ABSTRACT:
For the production of hetero-bispecific monoclonal antibodies at least the genes for the light chain and for the variable part of the heavy chain are isolated from a hybridoma cell line which secretes an antibody with a desired specificity and they are inserted into a eukaryotic plasmid vector which contains a marker capable of selection together with a strong promoter, this expression vector is transfected into a hybridoma cell line which secretes antibodies with a second desired specificity, the cell line is cultured, the antibodies are obtained and the bispecific antibody is isolated.

REFERENCES:
patent: 4474893 (1984-10-01), Reading et al.
Choo et al. DNA vol. 5(6) 529-537 (1986) Vectors for Expression and Amplification of cDNA Morrison et al., PNAS 81:6851-6855 (1984) Chimeric human antibody molecules . . . .

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