Method for co-amplification of human protein C genes in human ce

Details Method for co-amplification of human protein C genes in human ce Method for co-amplification of human protein C genes in human ce

1994-03-09

1996-08-27

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 911, 435 912, 4351723, 4353201, 536 231, 935 78, 935 88, C12N 1563, C12P 2100

Patent

active

055500366

ABSTRACT:
The present invention is a method of using the BK enhancer in tandem with a eukaryotic promoter to promote transcription of DNA that encodes a useful substance. The method of the present invention requires the presence of the E1A gene product for maximum expression of the useful substance. The present invention also comprises a number of useful expression vectors that comprise the BK enhancer in tandem with the adenovirus 2 late promoter positioned to drive expression of a variety of proteins, such as protein C, chloramphenicol acetyltransferase, and tissue plasminogen activator. The present invention further comprises a method for increasing the activity of the BK enhancer involving placement of the BK enhancer immediately upstream of the eukaryotic promoter used in tandem with the BK enhancer to drive expression of a useful substance. Furthermore, the present invention also comprises a method for coamplification of genes in primate cells. Additionally, the invention further comprises the recombinant human protein C molecule produced in 293 cells which comprises novel glycosylation patterns.

REFERENCES:
patent: 4562155 (1985-12-01), Ricciardi et al.
patent: 4775624 (1988-10-01), Bang et al.
patent: 4959318 (1990-09-01), Foster et al.
patent: 4965199 (1990-10-01), Capon et al.
Yang and Wu, 1979, Science 206:456-462.
Seif et al., 1979, Cell 18:963-977.
Rosenthal et al., 1983, Science 222:749-755.
Milanesi et al., 1984, Molecular and Cellular Biology 4(8):1551-1560.
Khoury and Gruss, 1983, Cell 33:313-314.
Borrelli et al., 1984, Nature 312:608-612.
Velcich and Ziff, 1985, Cell 40:705-716.
Hen et al., 1985, Science 230:1391-1394.
Wood et al., 1984, Nature 112:330-337.
Zain et al., 1979, Cell 16:851-861.
Solnick, 1981, Cell 24:135-143.
Berkner and Sharp, 1985, Nuc. Acids Res., 13(3):841-857.
Kaufman, 1984, Proc. Natl. Acad. Sci. USA 82:689-693.
Mansour et al., 1985, Proc. Natl. Acad. Sci. USA 82:1359-1363.
Davis et al., 1985, Proc. Natl. Acad. Sci. USA 82:7560-7564.
Wasylyk et al., 1983, Cell 32:503-514.
Imperiale et al., 1983, Cell 35:127-136.
Green et al., 1983, Cell 35:137-148.
Gaynor et al., 1984, Proc. Natl. Acad. Sci. USA 81:1193-1197.
Alwine, 1985, Mol. and Cell. Biol. 5(5):1034-1042.
Lewis and Manley, 1985, Nature 317:172-175.
Lebkowski et al., 1985, Nature 317:169-171.
Weeks and Jones, 1985, Nuc. Acids Res. 13(14):5389-5402.
Leff and Chambon, 1986, Mol. and Cell. Biol. 6(1):201-208.
Foster et al. (1984) Proc. Natl. Acad. Sci (USA), vol. 81, pp. 4766-4770.

Affiliated with

Also associated with

Rating

Method for co-amplification of human protein C genes in human ce does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for co-amplification of human protein C genes in human ce, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for co-amplification of human protein C genes in human ce will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1055789