Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1992-07-31
1994-07-26
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
436537, 436534, 435 25, 435 71, 435 19, 435188, 435810, 536 41, 536 172, 536 179, C12Q 128, G01N 33542, G01N 33536
Patent
active
053326628
ABSTRACT:
Methods and compositions are disclosed for determining a peroxidatively active substance (PAS). The methods comprise the step of detecting a fluorescent signal produced upon cleavage of a compound of the formula F-L-Q, wherein F is a fluorester capable of producing the signal, Q is a quencher capable of quenching the signal when linked to F, and L is a bond, or a linking group having a bond, wherein the bond is capable of being cleaved by a reaction of the PAS with a substrate of the PAS and a hydrogen donor wherein the cleavage of the bond substantially reduces the quenching. The methods have application in a wide variety of systems including assays and improved assays for analytes. Also disclosed are kits for conducting the methods and improvements in accordance with the present invention.
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Bosse Mark L.
Leary Louise N.
Precivale Shelley G.
Syntex (U.S.A.) Inc.
Wityshyn Michael G.
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