Thiobenzoylation method of peptide sequencing with gas chromatog

Details Thiobenzoylation method of peptide sequencing with gas chromatog Thiobenzoylation method of peptide sequencing with gas chromatog

1992-07-24

1993-09-21

Housel, James C.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

436 92, 436 96, 436161, 436173, 530345, 530410, G01N 3072, G01N 3368

Patent

active

052468658

ABSTRACT:
A protein microsequencing method for use in conjunction with the thiobenzoylation degradation of polypeptides and proteins is disclosed. The process involves reaction of the N-terminal amino acid of a polypeptide with an excess of a thiobenzoylating reagent. The derivatized polypeptide is subjected to cleavage by acid which forms a 4-substituted 2-phenyl-5(4H) thiazolone. The thiazolone is acylated to form a 5-acyloxy-2-phenylthiazole and subjected to detection by both gas chromatography and chemical ionization mass spectroscopy.

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Previero, A. "Alternative Reagents in Sequential Degradation on Solid Phase Supports" in: Hirs, C. H. W. Methods in Eznymology, vol. 47, Part E, pp. 289-299 (1977).
Barrett, G. C. et al. "Thin Layer Chromatography of N-Thiobenzoylamino Acid Analides" Journal of Chromatrography of N-Thiobenzoylamino Acid Analides Journal of Chromatography, vol. 39 (1969) pp. 47-52.
Vance, D. E. et al., "Reaction of Proteins and Peptides with Methyl isothiocyanate and Identification of the Methylthiohydantoins by Gas-Liquid Chromatography" Chemical Abstracts, vol. 73 (1970) No. 5270k.

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