Method for detecting bioindividuals by use of nonnatural type nu

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 6, 435911, 436 94, 436164, 436172, 436800, 536 231, 536 237, 536 2372, 536 2374, 536 2432, 935 1, 935 5, 935 8, 935 77, 935 78, C12Q 170, C12Q 168, C07H 2104, C12N 1500

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055455212

ABSTRACT:
A method for detecting live individuals comprises a step of reacting a sample with a probe having a nonnatural type nucleic acid and a label substance linked with the nucleic acid, which nonnatural type nucleic acid contains a base sequence complementary to a base sequence of a target nucleic acid in the live target individuals to be detected and which probe is able to be incorporated into the live target individuals; and detecting the probe incorporated into the live target individuals by the utilization of the label substance of the probe when the live target individuals are present in the sample. A probe has a nonnatural type nucleic acid and a label substance linked with the nucleic acid, which nonatural type nucleic acid contains a base sequence complementary to a base sequence of a target nucleic acid in a live target individuals to be detected, and the probe is able to be incorporated into the live target individuals.

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patent: 4544546 (1985-10-01), Wang et al.
patent: 5248671 (1993-09-01), Smith
Yu et al., "Sensitive Detection of RNAs in Single Cells by Flow Cytometry", Nucleic Acids Research, vol. 20, No. 1, pp. 83-88, 1991.

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