Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1994-03-15
1996-08-13
Chan, Christina Y.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 18, 435808, 436 73, 436164, 436172, 436805, 422 55, 422 827, 422 828, 422 8211, 356 731, 356301, C12Q 100, G01N 2100
Patent
active
055455174
ABSTRACT:
The present invention is a process and apparatus for metal ion detection. The process of the invention has the steps of (1) disposing, in an analyte medium, a macromolecule suitable for selective complexation with the target metal ion species; (2) disposing, in the analyte medium, an appropriate photoluminescent indicator that will emit in a measurably different manner when bound to the metallomacromolecule complex, compared with its unbound state; (3) exciting the photoluminescent indicator species; and (4) monitoring the emission of the photoluminescent indicator species to detect changes in its emission. The apparatus of the invention has (1) a macromolecule suitable for selective complexation with the target metal ion species disposed in an analyte medium; (2) an appropriate photoluminescent indicator that will emit in a measurably different manner when bound to the metallomacromolecule complex, compared with its unbound state, also disposed in the analyte medium; (3) a source for exciting the photoluminescent indicator species; and (4) a detector for monitoring the emission of the photoluminescent indicator species to detect changes in its emission.
REFERENCES:
patent: 5141312 (1992-08-01), Thompson et al.
Chen et al, The Journal of Biological Chemistry, vol. 242, No. 24, pp. 585823, 1967.
Thompson et al, Anal. Chem., vol. 65, No. 7, pp. 853-856, 1993.
James et al, Chemical Abstracts, vol. 92, pp. 647, Ref. #31841d, 1980.
Lieberman et al, Chemical Abstracts, vol. 107, pp. 423-424, Ref. #204814f, 1987.
Liberman et al, Chemical Abstracts, vol. 114, pp. 907-908, Ref. #198618w, 1991.
Thompson et al., "Component Selection for Fiber-Optic Fluorometry", Applied Spectroscopy 44 (1) 117-22 (1990).
Szmacinski et al., "Optical measurements of pH using fluorescence Lifetimes and phase-modulation fluorometry", Analytical Chem. 65 (13) 1668-74 (1993).
Thompson et al., "Phase Fluorimetric Method for Determination of Standard Lifetimes", Analytical Chem. 60 (7) 670-74 (1988).
Thompson et al., "Enzyme-based Fiber Optic Zinc Biosensor", Analytical Chem. 65 (6) 730-34 (1993).
Thompson et al., "Fiber Optic pH Sensor Based on Phase Fluorescence Lifetimes", Analytical Chem. 65 (7) 853-56 (1993).
Golden et al., "Fluorometer and tapered fiber optic probes for sensing in the evanescent wave", Optical Eng'g 31 (7) 1458-62 (1992).
Lakowicz et al., "Frequency-domain fluorescence spectroscopy", in Topics in Flourescence Spectroscopy vol. 1: Techniques, (J.R. Lakowicz, ed.) pp. 293-336 (Plenum Press 1991).
Thompson, "Flourescence-Based Fiber Optic Sensors", in Topics in Flourescence Spectroscopy vol. 2: Principles (J.R. Lakowicz, ed.) pp. 345-365 (plenum Press 1991).
Bruland, "Complexation of zinc by natural organic ligands in the central North Pacific", Limnology and Oceanography 34 (2) 269-85 (1989).
Sherrell et al., "Zinc, chromium, vanadium and iron in the Mediterranean Sea", Deep-Sea Research, 35 (8) 1319-34 (1988).
Jones Eric R.
Thompson Richard B.
Chan Christina Y.
Karasek John J.
McDonnell Thomas E.
Mohamed Abdel A.
The United States of America as represented by the Secretary of
LandOfFree
Selective metal ion detection using a photoluminescent indicator does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Selective metal ion detection using a photoluminescent indicator, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Selective metal ion detection using a photoluminescent indicator will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1047239