Method for increasing specificity in competitive immunoassays

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 793, 436518, 436820, 436825, 4241391, 4241491, 4241611, C12Q 170

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active

056816952

ABSTRACT:
The invention is an improved immunoassay and method for detection of antibody to hepatitis B core antigen (anti-HBc). The improved assay comprises the addition of a reducing agent to decrease the number of false positive reactions in the assay.

REFERENCES:
patent: 4234564 (1980-11-01), McAleer et al.
patent: 4683136 (1987-07-01), Milich et al.
Davis et al, "Immunology An Introduction to Molecular and Cellular Principles of the Immune Responses", Second Ed, Copyright 1974, Harper & Row, p. 410.
J.W. Prahl, Biochem J., vol. 105, "The C-Terminal Sequences of the Heavy Chains of Human ImmunoglobulinG Myeloma Proteins of Differing Isotypes and Allotypes", 1967, pp. 1019-1028.
Slobin et al., The Journal of Biological Chemistry, vol. 243, No. 8, "The Specific Cleavage of Immunoglobulin Polypeptide Chains at Cysteinyl Residues", 1968, pp. 1777-1786.
Product Insert, Abbott Laboratories, "Corzyme.RTM." Enzyme Immunoassay for the Detection of Antibody to Hepatitis B Core Antigen in Serum or Plasma (Copyright, 1987).
Product Insert, Abbott Laboratories, "CobraB.RTM." Radioimmunoassay for the Detection of Antibody to Hepatitis B Core Antigen in Serum or Plasma (Copyright, 1987).
G. Caspari, et al., Unsatisfactory Specificities and Sensitivities of Six Enzyme Immunoassays for Antibodies to Hepatitis B Core Antigen J. Clin. Micro 27(9):2067-2072 (1989).
J.A. Weare, et al., Improvement in the Specificity of Assays for Detection of Antibody to Hepatitis B Core Antigen, J. Clin. Micro 29(3):600-604 (1991).
A.M. Spronk, et al., Improvements in Detection of Antibody to Hepatitis B Core Antigen by Treating Specimens with Reducing Agent in an Automated Microparticle Enzyme Immunoassay. J. Clin. Micro 29(3):611-616 (1991).
E.F. Robertson, et al., Characterization of a Reduction-Sensitive Factor From Human Plasma Responsible for Apparent False Activity in Competitive Assays for Antibody to Hepatitis B Core Antigen, J. Clin. Micro 29(3):605-610 (1991).
R.H. Yolken, et al., "Analysis of Nonspecific Reactions in Enzyme-Linked Immunosorbent Assay Testing For Human Rotavirus." J. Clin. Micro. 10(6):703-707 (1979).
C.L. Cambiaso, et al., J. Immunol. Methods 28:13-23 (1979).
R. Deyo, et al., Biol. Abstracts 70: Abstract No. 64854 (1980).
G.L. Angarano, et al., Biol. Abstracts 72: Abstract No. 32079 (1981).
H. Shiraishi, et al., Biol Abstracts 80: Abstract No. 23240 (1985).
Clin. Chem. 70:1474-1479 (1983).
M. Sallberg, et al., J. Clin. Micro. 27(5):849-853 (1989).

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