Chemistry: physical processes – Physical processes – Crystallization
Patent
1979-11-02
1981-12-29
Marcus, Michael S.
Chemistry: physical processes
Physical processes
Crystallization
252408, 422 61, G01N 3352
Patent
active
043080278
ABSTRACT:
A direct method for determination of iron in blood serum comprising reacting the blood serum with ferrozine in the presence of a reducing agent to bring iron ion into divalent form, thiosemicarbazide and hydrochloric acid at a pH between 1.7 and 2.1, without any addition of buffers and tensioactive agents and determining colorimetrically the iron content of the specimen by means of the colored complex formed between iron ion and ferrozine against a reagent blank.
REFERENCES:
Ruutu, Det. of Iron & Unsaturated Iron-Binding Capacity in Serum with Ferrozine, Clin. Chim. Acta. 61 (1975), 229-232.
Rice et al., Clin. Chim. Acta. 53 (1974), 391-393.
Yee et al., Clin. Chem., vol. 17, No. 9, 1971.
Duffy et al., Clin. Biochem. 10(3), 122-123 (1977).
Henry et al., Clinical Chem., Prin. & Techniques, 1974, Harper & Row (1974), pp. 679-681.
Stookey, Ferrozine-A New Spectrophotometric Reagent for Iron, Anal. Chem., vol. 42, No. 7, Jun. 1970, 779-781.
Carter et al., Spectrophotometric Det. of Serum Iron at the Submicrogram Level with a New Reagent (Ferrozine), Anal. Biochem. 40, 450-458 (1971).
Persijn et al., Det. of Serum Iron & Latent Iron-Binding Capacity (LIBC), Clin. Chim. Acta. 35 (1971), 91-98.
Improved Direct Specific Det. of Serum Iron & Total Iron-Binding Capacity, Clin. Chem., vol. 26, No. 1980, Ceriotti et al.
White et al., Clin. Chem. 19/51, 526-528 (1973).
Chem. Abstracts 83 (1975), #74953y, Spectrophotometric Determination of Serum Iron Without Deproteinization, Bellina et al.
Narikawa et al., Chem. Abst., vol. 83 (1975), #128398s, Serum Iron Deter. by Fekit.
Feigl; Chem. of Specific Selective & Sensitive Reactions, Acad. Press, 1949, pp. 56-57.
Johnson M. R.
Marcus Michael S.
R.C.C. Societa' Ricerche di Chimica Clinica S.r.l.
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