Means for testing for pregnancy

Details Means for testing for pregnancy Means for testing for pregnancy

1976-05-20

1978-06-13

Padgett, Benjamin R.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 23259R, A61K 4300, A61K 3900

Patent

active

040949630

ABSTRACT:
Disclosed is a method for the determination of the hormone human chorionic gonadotropin (HCG), luteinizing hormone (LH), prolactin (PRL) and HCG-like material in an aqueous sample comprising contacting a highly specific receptor for these hormones with an aqueous sample to be tested, providing a means of indicating whether binding has taken place between the receptor and hormone possibly contained the sample and observing the indicating means to determine the presence of the hormone in the sample. PRL may be determined separately or simultaneously with the HCG and LH. The receptor is a plasma membrane extract from the corpus luteum of an animal which possesses the receptor for HCG, LH, PRL and HCG-like material. Radioassay indicating means are preferred, according to which competitive protein binding between radioisotopically labeled hormone and hormone present in the sample is radiologically determined. The method has particular application to the determination to pregnancy in the human female.

REFERENCES:
patent: 3899298 (1975-08-01), Szczesniak
patent: 3988429 (1976-10-01), Richards et al.
Haour et al., J. Biol. Chem., 249(7), 2195-2205, (1974).
Menon et al., Biochem and Biophys. Res. Comm., 56(2), (1974).
Skelley et al., Clin. Chem., 19(2) (1973).
Rao et al., Biochemica et Biophisica Acta, 313, 372-389, (1973).

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