Genomic sequencing method

Details Genomic sequencing method Genomic sequencing method

1987-11-13

1991-11-12

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 436501, 436 94, 436173, 436174, 436175, 935 77, 935 78, C12Q 168, C12P 1934, G01N 33566, G01N 3348

Patent

active

050647547

ABSTRACT:
A method of determining the nucleotide sequence of a DNA molecule of arbitrary length as a single procedure by sequencing portions of the molecule in a fashion such that the sequence of the 5' end of the succeeding contiguous portion is sequenced as the 3' end of its preceeding portion is sequenced, for all portions, where the order of contiguous portions is determined by the sequence of the DNA molecule. Sequencing of the individual portions is accomplished by generating a family of polynucleotides under conditions which determine that the elements are partial copies of the portion and are of random nucleotide length on the 3' and 5' ends about a dinucleotide which is an internal reference point; determining the base composition and terminal base identity of each element of the family and solving for the sequence by a method of analysis wherein the base composition and terminal base data of each element is used to solve for a single base of the sequence by assigning the base to either the 5' or 3' end of the partial sequence about the internal reference point as the entire sequence of the portion is built up from a dinucleotide.

REFERENCES:
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Budzikiewicz, H. and Linschied, N., "Sequential Analysis of Oligonucleotides by Mass Spectrometry," Adv. Mass Spectrum 13, , 1500.
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Freyell, J., "A Possible Electron Dense Label for Use in the Sequencing of DNA by Electron Microscopy," J. Theor. Biol. 59:241-242, (1976).
Rose, S. D., "Mercuration of Modified Nucleotides: Chemical Methods Toward Nucleic Acid Sequencing by Electron Microscopy," Biochem. Biophys. Act. 361: 231-235 (1974).
Gross, M. L. et al., "Mass Spectral Studies of Probe Pyrolysis of Intact Oligoribonucleation," Nucleic Acids Res. 5:2695-2704 (1978).
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