Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal
Patent
1984-12-06
1986-02-25
Schain, Howard E.
Chemistry of carbon compounds
Miscellaneous organic carbon compounds
C-metal
2601125R, 424 85, 435811, C07G 700, A61K 4502
Patent
active
045727988
ABSTRACT:
Reduced cysteine-containing proteins consisting of recombinant IFN-.beta., IL-2 or muteins thereof may be oxidized selectively so that the recombinant proteins have essentially the same disulfide bridging and biological activity as their native counterparts. The oxidized product is substantially free of unwanted side products and contains a minimal amount of intermolecular oligomers. The oxidation takes place in an aqueous medium containing a solubilizing agent at a pH of about 5.5 to 9, preferably at a pH of about 7. The reaction is initiated by addition of at least an effective amount of an oxidation promoter containing a Cu.sup.+2 cation such as CuCl.sub.2 or o-phenanthroline/Cu.sup.+2 complex in the presence of air.
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Halenbeck Robert F.
Koths Kirston E.
Cetus Corporation
Halluin Albert P.
Hasak Janet E.
Schain Howard E.
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